Department of Chemistry and Biochemistry, Islamic Azad University , Neyshabur Branch, Neyshabur, Iran.
Nucleic Acid Ther. 2011 Oct;21(5):355-8. doi: 10.1089/nat.2011.0314.
N(6)-Isopentenyladenosine (iPA), a member of the cytokinin family of plant hormones, exerts remarkable inhibition on tumor cell proliferation and apoptosis in several tumor cell lines. In this study, we report that iPA is able to inhibit the proliferation and promotes apoptosis in HCT-15 human colon cancer cells in a dose-dependent manner with a concentration of 2.5 μM, which causes 50% inhibition of cell viability. The cell cycle analysis by flow cytometry showed that iPA-induced growth arrest could be associated to apoptosis. Moreover, suppression of clonogenic activity occurs after exposure to iPA at a concentration of 2.5 μM for HCT-15.
N(6)-异戊烯基腺嘌呤（iPA）是植物激素细胞分裂素家族的一员，对几种肿瘤细胞系中的肿瘤细胞增殖和凋亡有显著的抑制作用。在这项研究中，我们报告 iPA 能够以 2.5μM 的浓度剂量依赖性地抑制 HCT-15 人结肠癌细胞的增殖并促进其凋亡，该浓度可使细胞活力抑制 50%。通过流式细胞术的细胞周期分析表明，iPA 诱导的生长停滞可能与凋亡有关。此外，在浓度为 2.5μM 的 iPA 作用下，HCT-15 的集落形成活性受到抑制。
