Department of Physics and Astrophysics, University of Delhi, Delhi 110007, India.
Biosens Bioelectron. 2012 Oct-Dec;38(1):11-8. doi: 10.1016/j.bios.2012.03.043. Epub 2012 Apr 20.
An efficient reagentless uric acid biosensor has been realized using a copper oxide (CuO) thin film matrix grown onto platinum (Pt) coated corning glass substrates by pulsed laser deposition (PLD) technique. The p-type CuO matrix successfully introduces redox property in the electrode and provides enhanced electron communication features. Sensing response obtained by the bioelectrocatalytic oxidation of uric acid by uricase/CuO/Pt/glass electrode was studied without any external mediator using cyclic voltammetry (CV) and photometric assay. The studies reveal that the uricase/CuO/Pt/glass bio-electrode exhibits good linearity over a wide range of 0.05 mM to 1.0mM uric acid concentration with enhanced response of 2.7 mA/mM and high shelf life (>14 weeks). A low Michaelis-Menten constant (K(m)) of 0.12 mM, indicate that the immobilized enzyme (uricase) has enhanced affinity towards its analyte (uric acid). The results confirm promising application of the p-type CuO thin film matrix for the realization of a reagentless integrated implantable biosensor.
已使用脉冲激光沉积 (PLD) 技术在涂覆有铂 (Pt) 的康宁玻璃基板上生长的氧化铜 (CuO) 薄膜基质实现了一种高效无试剂尿酸生物传感器。p 型 CuO 基质成功地在电极中引入氧化还原性质，并提供增强的电子通信特性。通过循环伏安法 (CV) 和分光光度法研究了尿酸酶/CuO/Pt/玻璃电极对尿酸的生物电化学氧化的传感响应，而无需任何外部介质。研究表明，尿酸酶/CuO/Pt/玻璃生物电极在 0.05 mM 至 1.0 mM 的尿酸浓度范围内具有良好的线性范围，响应增强为 2.7 mA/mM，保质期长 (>14 周)。低米氏常数 (K(m)) 为 0.12 mM，表明固定化酶（尿酸酶）对其分析物（尿酸）具有增强的亲和力。结果证实 p 型 CuO 薄膜基质在实现无试剂集成植入式生物传感器方面具有广阔的应用前景。
