Department of Physics and Astrophysics, University of Delhi, Delhi 110007, India.
Biosens Bioelectron. 2011 Dec 15;30(1):333-6. doi: 10.1016/j.bios.2011.09.026. Epub 2011 Sep 29.
Present study highlights the importance of RF sputtered NiO thin film deposited on platinum coated glass substrate (NiO/Pt/Ti/glass) as a potential matrix for the realization of highly sensitive and selective uric acid biosensor. Uricase has been immobilized successfully onto the surface of NiO matrix by physical adsorption technique. The prepared bioelectrode (uricase/NiO/Pt/Ti/glass) is utilized for sensing uric acid using the cyclic voltammetry and UV visible spectroscopy techniques. The bioelectrode is found to exhibit highly efficient sensing response characteristics with high sensitivity of 1278.48 μA/mM; good linearity of 0.05-1.0 mM, and very low Michaelis-Menten constant (k(m)) of 0.17 mM indicating high affinity of uricase towards the analyte. The enhanced response is due to the development of NiO matrix with good electron transport property and nanoporous morphology for effective loading of enzyme with preferred orientation.
本研究强调了射频溅射在铂涂覆玻璃基底（NiO/Pt/Ti/glass）上沉积的 NiO 薄膜作为实现高灵敏度和选择性尿酸生物传感器的潜在基质的重要性。尿酸酶已通过物理吸附技术成功固定在 NiO 基质表面。所制备的生物电极（尿酸酶/NiO/Pt/Ti/glass）用于通过循环伏安法和紫外可见光谱技术检测尿酸。发现生物电极具有高效的传感响应特性，具有 1278.48 μA/mM 的高灵敏度；0.05-1.0 mM 的良好线性度和非常低的米氏常数（k(m)）为 0.17 mM，表明尿酸酶对分析物具有高亲和力。增强的响应归因于 NiO 基质的发展，其具有良好的电子传输性能和纳米多孔形态，可有效负载具有优选取向的酶。
