2008 年，伊朗南部霍尔木兹甘省贾斯克县皮肤利什曼病患者分离的利什曼原虫的分子检测。
Molecular detection of Leishmania isolated from cutaneous leishmaniasis patients in Jask County, Hormozgan Province, Southern Iran, 2008.
机构信息
Department of Medical Entomology, Schools of Health and Nutrition, Shiraz University of Medical Sciences, Shiraz, Iran.
出版信息
Asian Pac J Trop Med. 2012 Jul;5(7):514-7. doi: 10.1016/S1995-7645(12)60090-X.
OBJECTIVE
To investigate on patients leishmanial infections in Jask County.
METHODS
Impression smears were prepared from patients in 2008, all, were chequed for leishmanial infection by microscopy and molecular assays. Whole DNA was extracted using Proteinase K and Phenol/Chloroform/Isoamyl alcohol method. The variable segment on minicircles of kinetoplast DNA was amplified via a Nested-PCR technique using species-specific primers (LIN R4-LIN 17 -Lin 19).
RESULTS
A total of 40 smears were prepared from 20 patients, from which, eight samples (40%) were positive for leishman body by microscopic method, while, 18 samples (90%) were positive, molecularly. The parasite was identified as Leishmania major (L. major).
CONCLUSION
Zoonotic or Rural cutaneous leishmaniasis is endemic in Jask County whose pathogen is L. major. Molecular assays using specific primers are very accurate and more sensitive and specific than microscopy which is time consuming and needs master microscopists.
目的
调查贾斯克县的利什曼原虫病患者。
方法
2008 年，从所有患者中制备了压片，通过显微镜检查和分子检测检查所有压片是否存在利什曼原虫感染。使用蛋白酶 K 和酚/氯仿/异戊醇法提取全 DNA。使用种特异性引物（LIN R4-LIN 17 -Lin 19）通过巢式 PCR 技术扩增小环动质体 DNA 的可变片段。
结果
从 20 名患者中总共制备了 40 个涂片，其中 8 个样本（40%）通过显微镜法呈利什曼体阳性，而 18 个样本（90%）分子法阳性。寄生虫被鉴定为利什曼原虫（L. major）。
结论
贾斯克县流行的是动物源性或农村皮肤利什曼病，病原体是 L. major。使用特异性引物的分子检测非常准确，比需要熟练显微镜师且耗时的显微镜检查更敏感和特异。
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