Laboratoire IMAGES EA 4218, University of Perpignan Via Domitia, Perpignan, France.
Anal Bioanal Chem. 2012 Aug;404(3):711-20. doi: 10.1007/s00216-012-6092-6. Epub 2012 May 31.
The inhibition characteristics of three different protein phosphatases by three microcystin (MC) variants--LR, YR, and RR--were studied. The corresponding K (I) for each enzyme-MC couple was calculated. The toxicity of MC varies in the following order: MC-LR > MC-YR > MC-RR. The sensitivity of the enzymes increased in the following order: mutant PP2A < mutant PP1 < natural PP2A. The best limit of detection obtained was 21.2 pM MC-LR using the most sensible enzyme. Methanol, ethanol, and acetonitrile up to 2 % (v/v) may be used in inhibition measurements. An artificial neural network (ANN) was used to discriminate two MC variants--LR and YR--using the differences in inhibition percentages measured with mutant PP1 and natural PP2A. The ANN is able to analyze mixtures with concentrations ranging from 8 to 98 pM MC-LR and 31 to 373 pM MC-YR.
研究了三种不同的微囊藻毒素（MC）变体-LR、YR 和 RR-对三种不同蛋白磷酸酶的抑制特性。计算了每种酶-MC 偶联物的相应 K（I）。MC 的毒性按以下顺序排列：MC-LR > MC-YR > MC-RR。酶的灵敏度按以下顺序增加：突变型 PP2A < 突变型 PP1 < 天然 PP2A。使用最敏感的酶，获得的最佳检测极限为 21.2 pM MC-LR。甲醇、乙醇和乙腈最高可达 2%（v/v）可用于抑制测量。人工神经网络（ANN）用于使用突变型 PP1 和天然 PP2A 测量的抑制百分比差异来区分两种 MC 变体-LR 和 YR。ANN 能够分析浓度范围为 8 到 98 pM MC-LR 和 31 到 373 pM MC-YR 的混合物。
