细胞肿胀抑制灌注大鼠肝脏中的蛋白水解作用。
Cell swelling inhibits proteolysis in perfused rat liver.
作者信息
Häussinger D, Hallbrucker C, vom Dahl S, Lang F, Gerok W
机构信息
Medizinische Universitätsklinik, Freiburg, Federal Republic of Germany.
出版信息
Biochem J. 1990 Nov 15;272(1):239-42. doi: 10.1042/bj2720239.
Abstract
Exposure of isolated single-pass-perfused rat liver to hypo-osmotic media resulted in liver cell swelling and an inhibition of release of branched-chain amino acids. Similarly, cell swelling inhibited [3H]leucine release from perfused livers from rats in which liver proteins were prelabelled in vivo by intraperitoneal injection of L-[4,5-3H]leucine 16-20 h before the experiment. The effects of cell swelling on [3H]leucine release were fully reversible. [3H]Leucine release was also inhibited when cell swelling was induced by addition of glutamine (0.5-2 mM). There was a close relationship between the inhibition of [3H]leucine release and the degree of liver cell swelling, regardless of whether cell swelling was induced by hypo-osmotic perfusion or addition of glutamine. The data suggest that the known anti-proteolytic effect of glutamine is in large part due to glutamine-induced hepatocyte swelling.
摘要
将分离的单循环灌注大鼠肝脏暴露于低渗介质中会导致肝细胞肿胀，并抑制支链氨基酸的释放。同样，细胞肿胀抑制了实验前16 - 20小时通过腹腔注射L-[4,5-³H]亮氨酸在体内预先标记肝脏蛋白质的大鼠灌注肝脏中[³H]亮氨酸的释放。细胞肿胀对[³H]亮氨酸释放的影响是完全可逆的。当通过添加谷氨酰胺（0.5 - 2 mM）诱导细胞肿胀时，[³H]亮氨酸的释放也受到抑制。无论细胞肿胀是由低渗灌注还是添加谷氨酰胺诱导的，[³H]亮氨酸释放的抑制与肝细胞肿胀程度之间都存在密切关系。数据表明，已知的谷氨酰胺的抗蛋白水解作用在很大程度上归因于谷氨酰胺诱导的肝细胞肿胀。
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