Uzgiris E E
GE Corporate Research and Development Center, Schenectady, NY 12301.
Biochem J. 1990 Nov 15;272(1):45-9. doi: 10.1042/bj2720045.
Two distinct lattice structures are observed for two-dimensional (2-D) antibody organization on phospholipid films. A low-order, small-unit-cell, square lattice is obtained at pH 7 and below for mouse IgE, mouse IgG2a and IgG2b and rabbit IgG. At pH 7.5 and above, the observed lattice structure switches to a large-unit-cell, hexagonal type for rabbit IgG and mouse IgE. Interchain disulphide reduction by exposure to 2 mM-dithiothreitol results in the formation of the compact 2-D lattice for all cases and for all pH conditions.
在磷脂膜上观察到二维（2-D）抗体组织存在两种不同的晶格结构。对于小鼠IgE、小鼠IgG2a和IgG2b以及兔IgG，在pH 7及以下可获得一种低阶、小单位晶胞的方形晶格。在pH 7.5及以上，观察到的晶格结构转变为兔IgG和小鼠IgE的大单位晶胞六边形类型。通过暴露于2 mM二硫苏糖醇进行链间二硫键还原，在所有情况下和所有pH条件下都会形成紧密的二维晶格。
