Department of Women's and Children's Health, Institute of Translational Medicine, University of Liverpool, Alder Hey Children's NHS Foundation Trust Hospital, Eaton Road, Liverpool, L12 2AP, UK.
J Clin Immunol. 2012 Oct;32(5):1019-25. doi: 10.1007/s10875-012-9710-3. Epub 2012 May 31.
Sphingosine-1-phosphate (S1P) is an active sphingolipid with chemotactic abilities and has been linked to inflammatory mediators and autoimmune disease. The aim of this study was to assess whether children with juvenile-onset systemic lupus erythematosus (JSLE) express increased systemic and/or urinary concentrations of S1P.
A subgroup of patients participating in the UK JSLE Cohort Study, were invited to participate. Cross sectional serum and urine samples were prospectively collected along with demographic and standard clinical data. Results were compared to a cohort of disease controls (Henoch Schonlein Purpura; HSP) and healthy controls (HC).
The median age of JSLE patients (n = 15) was 13.6 years (7.2-16.9 years). The serum concentrations of S1P in JSLE patients (7.4 uM, IQR 6.3-12.3 uM) were statistically significantly increased when compared to patients with HSP (n = 10; 5.2 uM, IQR 4.0-7.9 uM; p = 0.016) and HCs (n = 10; 3.8 uM, IQR 2.1-5.8 uM; p = 0.003). There was a trend towards increased serum S1P concentrations between patients with active lupus nephritis (n = 8; 8.7 uM, IQR 6.2-15.3 uM) compared to lupus non-nephritis (n = 7; 6.6 uM, IQR 6.3-10.6 uM; p = 0.355). No relationship was found between disease activity markers and S1P. Urine S1P concentrations were no different between JSLE patients (56.0 nM, IQR 40.3-96.6 nM) and HCs (58.7 nM, IQR 0-241.9 nM; p = 0.889).
We have demonstrated, for the first time, an increased serum concentration of S1P in a cohort of JSLE patients. These findings highlight a role of S1P in the pathophysiology of JSLE that warrants further investigation.
神经鞘氨醇-1-磷酸（S1P）是一种具有趋化作用的活性鞘脂，与炎症介质和自身免疫性疾病有关。本研究旨在评估幼年特发性系统性红斑狼疮（JSLE）患儿是否存在全身性和/或尿液中 S1P 浓度升高。
邀请参加英国 JSLE 队列研究的部分患者参加。前瞻性收集血清和尿液标本，同时收集人口统计学和标准临床数据。将结果与疾病对照组（过敏性紫癜；HSP）和健康对照组（HC）进行比较。
JSLE 患者（n=15）的中位年龄为 13.6 岁（7.2-16.9 岁）。与 HSP 患者（n=10；5.2 uM，IQR 4.0-7.9 uM；p=0.016）和 HC（n=10；3.8 uM，IQR 2.1-5.8 uM；p=0.003）相比，JSLE 患者的血清 S1P 浓度统计学上显著升高。与狼疮肾炎患者（n=8；8.7 uM，IQR 6.2-15.3 uM）相比，狼疮非肾炎患者（n=7；6.6 uM，IQR 6.3-10.6 uM；p=0.355）的血清 S1P 浓度有升高趋势。S1P 与疾病活动标志物之间无相关性。JSLE 患者的尿液 S1P 浓度与 HC（56.0 nM，IQR 40.3-96.6 nM）无差异（58.7 nM，IQR 0-241.9 nM；p=0.889）。
我们首次证明，JSLE 患者的血清 S1P 浓度升高。这些发现提示 S1P 在 JSLE 的病理生理学中起作用，值得进一步研究。
