Surgery Clinic, University of Pecs, Pecs, Hungary.
J Mol Neurosci. 2012 Nov;48(3):667-73. doi: 10.1007/s12031-012-9815-4. Epub 2012 May 31.
Pituitary adenylate cyclase activating polypeptide (PACAP) is a pleiotropic and multifunctional neuropeptide having important roles in various physiological processes. Recent trends in PACAP research point to the clinical introduction of PACAP or its analogs/fragments possibly in the near future. Recently, we have shown the presence of PACAP in human plasma, milk, placenta, and follicular fluid samples. However, relatively few data are available on PACAP in human tissues from patients with different disorders. The aim of the present study was to determine, by radioimmunoassay, the tissue level of PACAP38-like immunoreactivity (LI) and PACAP27-LI in different primary non-small cell lung cancer, colon tumor samples, and in cardiac muscle samples from patients suffering from ischemic heart disease and valvular disorders. We also labeled the PAC1 receptors in human cardiac cells. All samples showed significantly higher PACAP38-LI compared with PACAP27-LI. We found significantly lower levels of PACAP38-LI and PACAP27-LI in tumoral and peripheral samples compared with normal healthy tissue in both lung and colon cancers. Further investigations are necessary to describe the exact function of PACAP in oncogenesis. We showed that PACAP38-LI and PACAP27-LI are significantly higher in ischemic heart diseases compared with valvular abnormalities, suggesting that PACAP might play a role in ischemic heart disorders.
垂体腺苷酸环化酶激活肽（PACAP）是一种多功能神经肽，在各种生理过程中具有重要作用。最近的 PACAP 研究趋势表明，PACAP 或其类似物/片段可能在不久的将来会被临床应用。最近，我们已经在人血浆、牛奶、胎盘和卵泡液样本中发现了 PACAP 的存在。然而，关于不同疾病患者的人体组织中 PACAP 的数据相对较少。本研究的目的是通过放射免疫分析法测定不同原发性非小细胞肺癌、结肠癌肿瘤样本和缺血性心脏病及瓣膜病患者心肌组织中 PACAP38 样免疫反应性（LI）和 PACAP27-LI 的组织水平。我们还标记了人心脏细胞中的 PAC1 受体。所有样本均显示 PACAP38-LI 显著高于 PACAP27-LI。我们发现肺癌和结肠癌中肿瘤和外周样本的 PACAP38-LI 和 PACAP27-LI 水平明显低于正常健康组织。需要进一步研究来描述 PACAP 在肿瘤发生中的确切功能。我们发现，与瓣膜异常相比，缺血性心脏病患者的 PACAP38-LI 和 PACAP27-LI 明显升高，这表明 PACAP 可能在缺血性心脏病中发挥作用。
