Department of Biochemistry and Molecular Biology II, University of Granada, Granada, Spain.
Mol Nutr Food Res. 2012 May;56(5):733-40. doi: 10.1002/mnfr.201100703.
Anti-atherogenic features of olive oil (OO) have been attributed, in part, to minor compounds, via diverse mechanisms, although its effects on the CD36 receptor have not been examined. We investigated the effects of minor compounds of OO (squalene (SQ), tyrosol (Tyr) and hydroxytyrosol (OH-Tyr)), on the expression of the CD36 receptor, as well as on monocyte/macrophage differentiation and proliferation.
U937 monocytic cells and macrophages (obtained with 10 nM phorbol-myristate-acetate) were exposed to Tyr, OH-Tyr or SQ at 0, 10, 75 and 200 μM with/without native or oxidised LDL(oxLDL). Flow cytometry was used to achieve the expression of CD36 in both cell types exposed to oxLDL plus antioxidants, as well as the inhibition of monocyte/macrophage differentiation after oxLDL and apoptosis. SQ caused a dose-dependent reduction of CD36 in the presence of native and moderate LDL in monocytes and macrophages. Phenotype-dependent cytotoxic and antiproliferative effects were found for OH-Tyr (p < 0.05), while SQ affected neither monocytes nor macrophages (p < 0.01).
SQ does not prevent monocyte migration and activation into macrophages, but it would inhibit oxLDL uptake by macrophages, by reducing CD36 expression. This study provides new data about the role of the components of OO in the prevention of atherosclerosis.
橄榄油（OO）的抗动脉粥样硬化特性部分归因于通过多种机制的微量化合物，尽管尚未研究其对 CD36 受体的影响。我们研究了 OO 的微量化合物（角鲨烯（SQ）、酪醇（Tyr）和羟基酪醇（OH-Tyr））对 CD36 受体表达以及单核细胞/巨噬细胞分化和增殖的影响。
U937 单核细胞和巨噬细胞（用 10 nM 佛波醇 12-肉豆蔻酸 13-乙酸酯诱导）在存在或不存在天然或氧化 LDL（oxLDL）的情况下，用 0、10、75 和 200 μM 的 Tyr、OH-Tyr 或 SQ 进行处理。使用流式细胞术来实现暴露于 oxLDL 和抗氧化剂的两种细胞类型中 CD36 的表达，以及 oxLDL 和细胞凋亡后单核细胞/巨噬细胞分化的抑制。在单核细胞和巨噬细胞中，存在天然和中等 LDL 时，SQ 会导致 CD36 的剂量依赖性降低。发现 OH-Tyr 具有表型依赖性细胞毒性和抗增殖作用（p<0.05），而 SQ 既不影响单核细胞也不影响巨噬细胞（p<0.01）。
SQ 不能阻止单核细胞迁移和激活为巨噬细胞，但它可以通过降低 CD36 表达来抑制巨噬细胞对 oxLDL 的摄取。这项研究提供了关于 OO 成分在预防动脉粥样硬化中的作用的新数据。
