Department of Materials Science & Engineering, University of Washington, Seattle, WA 98195, USA.
Macromol Biosci. 2012 Jul;12(7):882-92. doi: 10.1002/mabi.201100269. Epub 2012 May 30.
Chitosan-based fibrous matrices are prepared to mimic the ECM architecture and elucidate substrate-mediated hESC differentiation due to topographical scale and anisotropy without exogenic morphogens. Fibrous matrices support fewer pluripotent hESCs than films but enable topography-mediated hESC differentiation. Matrices composed of 400 nm and 1.1 µm diameter fibers support increased expression of neural markers indicative of ectodermal commitment while matrices of 200 nm diameter fibers increase expression of osteogenic and hepatic markers indicative of endodermal and mesodermal commitment. The fibrous-mediated hESC differentiation highlights the significant implication of tailored ECM-like substrates for hESC-based therapies.
壳聚糖基纤维基质被制备用来模拟细胞外基质的结构，并阐明由于形貌尺度和各向异性而无需外源性形态发生素的基质介导的 hESC 分化。纤维基质支持的多能 hESC 比薄膜少，但能够实现形貌介导的 hESC 分化。由 400nm 和 1.1μm 直径纤维组成的基质支持增加表达神经标记物，提示外胚层的定向；而由 200nm 直径纤维组成的基质增加表达骨形成和肝标记物，提示内胚层和中胚层的定向。纤维介导的 hESC 分化突出了定制的细胞外基质样底物对基于 hESC 的治疗的重要意义。
