College of Textiles, North Carolina State University, Raleigh, NC 27695, USA.
Macromol Biosci. 2012 Jul;12(7):893-900. doi: 10.1002/mabi.201100470. Epub 2012 May 30.
Functional PLA scaffolds are created with single component, core-sheath, or porous fiber morphology and doped with TCP nanoparticles to study the release profiles for use in bone tissue engineering applications. Pharmacokinetic analyses are performed for the three different nanofibrous structures after doping with TCP. Results indicate that single component and porous fiber scaffolds exhibit an initial-burst release profile whereas core-sheath fibers show a steady release. All scaffolds are then seeded with human adipose-derived stem cells (hASC), which remain viable and continue proliferation on all nanofibrous morphologies for up to 21 d. Osteogenic differentiation of hASC and cell-mediated calcium accretion are largest on porous fibers.
功能性 PLA 支架采用单组分、芯鞘或多孔纤维形态，并掺杂 TCP 纳米粒子，以研究在骨组织工程应用中的释放曲线。对掺杂 TCP 的三种不同纳米纤维结构进行药代动力学分析。结果表明，单组分和多孔纤维支架呈现初始突释释放曲线，而芯鞘纤维则呈现稳定释放。然后将所有支架接种人脂肪来源干细胞（hASC），这些细胞在所有纳米纤维形态上保持存活并持续增殖长达 21 天。多孔纤维上 hASC 的成骨分化和细胞介导的钙积累最大。
