Haimi Suvi, Suuriniemi Niina, Haaparanta Anne-Marie, Ellä Ville, Lindroos Bettina, Huhtala Heini, Räty Sari, Kuokkanen Hannu, Sándor George K, Kellomäki Minna, Miettinen Susanna, Suuronen Riitta
Regea Institute for Regenerative Medicine, University of Tampere, Tampere, Finland.
Tissue Eng Part A. 2009 Jul;15(7):1473-80. doi: 10.1089/ten.tea.2008.0241.
The aim of this study was to compare the effects of novel three-dimensional composite scaffolds consisting of a bioactive phase (bioactive glass or beta-tricalcium phosphate [beta-TCP] 10 and 20 wt%) incorporated within a polylactic acid (PLA) matrix on viability, distribution, proliferation, and osteogenic differentiation of human adipose stem cells (ASCs). The viability and distribution of ASCs on the bioactive composite scaffolds was evaluated using Live/Dead fluorescence staining, environmental scanning electron microscopy, and scanning electron microscopy. There were no differences between the two concentrations of bioactive glass and beta-TCP in PLA scaffolds on proliferation and osteogenic differentiation of ASCs. After 2 weeks of culture, DNA content and alkaline phosphatase (ALP) activity of ASCs cultured on PLA/beta-TCP composite scaffolds were higher relative to other scaffold types. Interestingly, the cell number was significantly lower, but the relative ALP/DNA ratio of ASCs was significantly higher in PLA/bioactive glass scaffolds than in other three scaffold types. These results indicate that the PLA/beta-TCP composite scaffolds significantly enhance ASC proliferation and total ALP activity compared to other scaffold types. This supports the potential future use of PLA/beta-TCP composites as effective scaffolds for tissue engineering and as bone replacement materials.
本研究的目的是比较由掺入聚乳酸(PLA)基质中的生物活性相(生物活性玻璃或β-磷酸三钙[β-TCP],含量分别为10%和20%重量)组成的新型三维复合支架对人脂肪干细胞(ASC)的活力、分布、增殖和成骨分化的影响。使用活/死荧光染色、环境扫描电子显微镜和扫描电子显微镜评估ASC在生物活性复合支架上的活力和分布。在PLA支架中,两种浓度的生物活性玻璃和β-TCP对ASC的增殖和成骨分化没有差异。培养2周后,与其他支架类型相比,在PLA/β-TCP复合支架上培养的ASC的DNA含量和碱性磷酸酶(ALP)活性更高。有趣的是,PLA/生物活性玻璃支架中的细胞数量显著更低,但ASC的相对ALP/DNA比率显著高于其他三种支架类型。这些结果表明,与其他支架类型相比,PLA/β-TCP复合支架显著增强了ASC的增殖和总ALP活性。这支持了PLA/β-TCP复合材料未来作为组织工程有效支架和骨替代材料的潜在用途。