现场评估一种简单的荧光方法，用于检测治疗随访期间痰标本中的活结核分枝杆菌。
Field evaluation of a simple fluorescence method for detection of viable Mycobacterium tuberculosis in sputum specimens during treatment follow-up.
机构信息
Epicentre, Paris, France.
出版信息
J Clin Microbiol. 2012 Aug;50(8):2788-90. doi: 10.1128/JCM.01232-12. Epub 2012 May 30.
Abstract
Simple tuberculosis (TB) treatment monitoring tools are needed. We assessed the performance of fluorescein-diacetate (FDA) smear microscopy for detection of viable Mycobacterium tuberculosis in sputum specimens (n = 288) of TB cases under treatment compared to culture (17.4% culture positivity). FDA sensitivity was moderate (83.7% [95% confidence interval {CI}, 70.3 to 92.6]), and specificity was low (66.1% [59.5 to 72.2]). The good negative predictive value (94.8% [90.1 to 97.8]) and negative likelihood ratio (0.2) suggest using this method to rule out treatment failure in settings without access to culture.
摘要
需要简单的结核病（TB）治疗监测工具。我们评估了荧光素二乙酸酯（FDA）痰涂片显微镜检测在治疗中的结核病例（n=288）中检测活结核分枝杆菌的性能，与培养（17.4%的培养阳性率）相比。FDA 的灵敏度为中度（83.7%[95%置信区间（CI）：70.3 至 92.6]），特异性较低（66.1%[59.5 至 72.2]）。良好的阴性预测值（94.8%[90.1 至 97.8]）和负似然比（0.2）表明，在无法进行培养的情况下，可使用该方法排除治疗失败的情况。
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