• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Field evaluation of a simple fluorescence method for detection of viable Mycobacterium tuberculosis in sputum specimens during treatment follow-up.现场评估一种简单的荧光方法，用于检测治疗随访期间痰标本中的活结核分枝杆菌。
J Clin Microbiol. 2012 Aug;50(8):2788-90. doi: 10.1128/JCM.01232-12. Epub 2012 May 30.
2
Visualizing viable Mycobacterium tuberculosis in sputum to monitor isolation measures.
J Infect. 2017 Feb;74(2):207-210. doi: 10.1016/j.jinf.2016.11.014. Epub 2016 Dec 7.
3
Fluorescein diacetate vital staining for detecting viability of acid-fast bacilli in patients on antituberculosis treatment.用于检测接受抗结核治疗患者中抗酸杆菌活力的二醋酸荧光素活体染色法。
Int J Mycobacteriol. 2016 Sep;5(3):294-298. doi: 10.1016/j.ijmyco.2016.06.003. Epub 2016 Jun 23.
4
Sputum monitoring during tuberculosis treatment for predicting outcome: systematic review and meta-analysis.肺结核治疗期间痰液监测对预测结局的作用：系统评价和荟萃分析。
Lancet Infect Dis. 2010 Jun;10(6):387-94. doi: 10.1016/S1473-3099(10)70071-2.
5
Light-emitting diode with various sputum smear preparation techniques to diagnose tuberculosis.发光二极管与各种痰涂片制备技术诊断结核病。
Int J Tuberc Lung Dis. 2012;16(3):402-7. doi: 10.5588/ijtld.10.0762.
6
Bulk acid-fast staining of sputum smears: time to end a taboo.痰涂片大量抗酸染色：是时候打破禁忌了。
Int J Tuberc Lung Dis. 2009 Sep;13(9):1119-23.
7
Pulmonary Tuberculosis Conversion Documented by Microscopic Staining for Detection of Dynamic, Dormant, and Dead Mycobacteria (DDD Staining).通过显微镜染色检测动态、休眠和死亡分枝杆菌（DDD染色）记录的肺结核转化
J Clin Microbiol. 2018 Sep 25;56(10). doi: 10.1128/JCM.01108-18. Print 2018 Oct.
8
Comparison of smear microscopy, culture, and real-time PCR for quantitative detection of Mycobacterium tuberculosis in clinical respiratory specimens.临床呼吸道标本中结核分枝杆菌定量检测的涂片显微镜检查、培养及实时荧光定量PCR比较
Scand J Infect Dis. 2013 Apr;45(4):250-5. doi: 10.3109/00365548.2012.727465. Epub 2012 Oct 31.
9
Use of light-emitting diode fluorescence microscopy to detect acid-fast bacilli in sputum.使用发光二极管荧光显微镜检测痰中的抗酸杆菌。
Clin Infect Dis. 2008 Jul 15;47(2):203-7. doi: 10.1086/589248.
10
A minimum 5.0 ml of sputum improves the sensitivity of acid-fast smear for Mycobacterium tuberculosis.至少5.0毫升痰液可提高结核分枝杆菌抗酸涂片的敏感性。
Am J Respir Crit Care Med. 2000 May;161(5):1559-62. doi: 10.1164/ajrccm.161.5.9908063.

引用本文的文献

1
Optimising fluorescein diacetate sputum smear microscopy for assessing patients with pulmonary tuberculosis.优化二乙酸荧光素痰涂片显微镜检查，以评估肺结核患者。
PLoS One. 2019 Apr 30;14(4):e0214131. doi: 10.1371/journal.pone.0214131. eCollection 2019.
2
Editorial commentary: dead or alive: can viability staining predict response to tuberculosis treatment?编者按：死还是活：活力染色能否预测结核病治疗反应？
Clin Infect Dis. 2015 Apr 15;60(8):1196-8. doi: 10.1093/cid/ciu1156. Epub 2014 Dec 23.
3
Clinical evaluation of tuberculosis viability microscopy for assessing treatment response.用于评估治疗反应的结核杆菌活力显微镜检查的临床评估
Clin Infect Dis. 2015 Apr 15;60(8):1186-95. doi: 10.1093/cid/ciu1153. Epub 2014 Dec 23.

本文引用的文献

1
Early and rapid microscopy-based diagnosis of true treatment failure and MDR-TB.基于显微镜检查的早期快速诊断真正的治疗失败和耐多药结核病。
Int J Tuberc Lung Dis. 2006 Nov;10(11):1248-54.
2
Yield of continued monthly sputum evaluation among tuberculosis patients after culture conversion.结核患者培养转阴后持续每月痰评估的阳性率
Int J Tuberc Lung Dis. 2002 Mar;6(3):238-45.
3
The significance of the persistent presence of acid-fast bacilli in sputum smears in pulmonary tuberculosis.肺结核患者痰涂片抗酸杆菌持续存在的意义
Chest. 1999 Sep;116(3):726-31. doi: 10.1378/chest.116.3.726.
4
Incidence and significance of acid-fast bacilli in sputum smears at the end of antituberculous treatment.抗结核治疗结束时痰涂片抗酸杆菌的发生率及意义。
Chest. 1996 Jun;109(6):1562-5. doi: 10.1378/chest.109.6.1562.
5
Acid-fast bacilli in sputum smears of patients with pulmonary tuberculosis. Prevalence and significance of negative smears pretreatment and positive smears post-treatment.肺结核患者痰涂片抗酸杆菌。涂片预处理阴性和治疗后涂片阳性的发生率及意义。
Am Rev Respir Dis. 1984 Feb;129(2):264-8.
6
Assessing the viability of Mycobacterium leprae by the fluorescein diacetate/ethidium bromide staining technique.通过荧光素二乙酸酯/溴化乙锭染色技术评估麻风分枝杆菌的生存能力。
Indian J Lepr. 1991 Apr-Jun;63(2):203-8.
7
[Application of FDA/EB staining for the detection of viable or non-viable mycobacteria in clinical specimens].[FDA/EB染色在临床标本中检测活菌或非活菌分枝杆菌的应用]
Kekkaku. 1992 Feb;67(2):113-7.

现场评估一种简单的荧光方法，用于检测治疗随访期间痰标本中的活结核分枝杆菌。

Field evaluation of a simple fluorescence method for detection of viable Mycobacterium tuberculosis in sputum specimens during treatment follow-up.

机构信息

Epicentre, Paris, France.

出版信息

J Clin Microbiol. 2012 Aug;50(8):2788-90. doi: 10.1128/JCM.01232-12. Epub 2012 May 30.

DOI:10.1128/JCM.01232-12
PMID:22649015
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3421534/
Abstract

Simple tuberculosis (TB) treatment monitoring tools are needed. We assessed the performance of fluorescein-diacetate (FDA) smear microscopy for detection of viable Mycobacterium tuberculosis in sputum specimens (n = 288) of TB cases under treatment compared to culture (17.4% culture positivity). FDA sensitivity was moderate (83.7% [95% confidence interval {CI}, 70.3 to 92.6]), and specificity was low (66.1% [59.5 to 72.2]). The good negative predictive value (94.8% [90.1 to 97.8]) and negative likelihood ratio (0.2) suggest using this method to rule out treatment failure in settings without access to culture.

摘要

需要简单的结核病（TB）治疗监测工具。我们评估了荧光素二乙酸酯（FDA）痰涂片显微镜检测在治疗中的结核病例（n=288）中检测活结核分枝杆菌的性能，与培养（17.4%的培养阳性率）相比。FDA 的灵敏度为中度（83.7%[95%置信区间（CI）：70.3 至 92.6]），特异性较低（66.1%[59.5 至 72.2]）。良好的阴性预测值（94.8%[90.1 至 97.8]）和负似然比（0.2）表明，在无法进行培养的情况下，可使用该方法排除治疗失败的情况。