Department of Chemistry, Moscow State University.
Acta Naturae. 2009 Jul;1(2):81-5.
Telomerase adds telomeric repeats to single-stranded DNA at the ends of the chromosomes. This enzyme is a ribonucleoprotein complex. Telomerase from yeast Saccharomyces cerevisiae consists of TLC1 RNA, which serves as a template for the synthesis of telomeric repeats, telomerase reverse transcriptase Est2p, and a number of accessory proteins (Est1p, Est3p, Ku70/Ku80, and Sm-complex). We found that the yeast telomerase complex contains a biotinylated component. The telomerase fraction containing biotinylated protein is active in vitro and constitutes a small part of the total amount of active telomerase isolated from cells. We speculate about the nature of the biotinylated component.
端粒酶将端粒重复序列添加到染色体末端的单链 DNA 上。这种酶是一种核糖核蛋白复合物。来自酿酒酵母的端粒酶由 TLC1 RNA 组成，它作为端粒重复序列合成的模板，端粒酶逆转录酶 Est2p 和许多辅助蛋白（Est1p、Est3p、Ku70/Ku80 和 Sm 复合物）。我们发现酵母端粒酶复合物含有一个生物素化的成分。含有生物素化蛋白的端粒酶部分在体外具有活性，并且构成从细胞中分离的总活性端粒酶的一小部分。我们推测生物素化成分的性质。
