霍乱弧菌毒素与NAD-琼脂糖的可逆结合。
Reversible binding of vibrio cholerae toxin on NAD-sepharose.
作者信息
Antoni G, Brizzi A, Casagli M C, Neri P
出版信息
Ital J Biochem. 1978 Nov-Dec;27(6):373-7.
PMID:226497
Abstract
When a crude or partially purified culture of Vibrio Cholerae is passed through a column of NAD-Sepharose, the skin toxic material is bound to the gel. Choleragen may be recovered by increasing the ionic strength of the elution buffer, as shown by the skin test, immunodiffusion against anti-choleragen antiserum and polyacrylamide gel electrophoresis. Affinity chromatography on NAD-Sepharose may be helpful for the concentration and purification of Choleragen.
摘要
当霍乱弧菌的粗培养物或部分纯化培养物通过NAD-琼脂糖柱时，皮肤毒性物质会与凝胶结合。通过增加洗脱缓冲液的离子强度可回收霍乱毒素，这通过皮肤试验、针对抗霍乱毒素抗血清的免疫扩散以及聚丙烯酰胺凝胶电泳得以证明。在NAD-琼脂糖上进行亲和层析可能有助于霍乱毒素的浓缩和纯化。
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