Ozbek Emin, Calik Gokhan, Otunctemur Alper, Aliskan Tamer, Cakir Suleyman, Dursun Murat, Somay Adnan
Okmeydani Training and Research Hospital, 1st Urology Clinic, Istanbul, Turkey.
Arch Ital Urol Androl. 2012 Mar;84(1):7-11.
To identify stem cell markers aldehyde dehydrogenase type 1 (ALDH-1) and nestin expression in renal cell carcinoma (RCC).
95 RCC patients who had radical or partial nephrectomy were included in this study. Normal renal tissue samples of the same patients were designated as the control group. Paraffin blocks of RCC patients were studied immunohistochemically. Expressions were analyzed semiquantitively. Relation of tumor grade and stage with these expressions was determined. Results were calculated with Mann Whitney U and Kruskal Wallis tests.
There were 55 male (57.8%) and 40 (42.2%) female patients in the study. Age and sex of the patients were not correlated with their antibody expressions (p > 0.05). ALDH-1 and nestin expression was higher in cancer tissues than normal tissues (p < 0.05). Nestin expression in renal cencer tissue was inversely related with tumor stage (p < 0.05) but there was no relation with Fuhrman grade. ALDH-1 expression was correlated with tumor grade (p < 0.05) but not with tumor stage (p > 0.05).
ALDH-land nestin expression have a role in RCC pathophysiology. There is a need to perform more studies about stem cell markers in RCC with more patients and in these future studies lymph node metastasis, distant organ metastasis and survival rates should be included.
鉴定肾细胞癌（RCC）中干细胞标志物醛脱氢酶1型（ALDH-1）和巢蛋白的表达情况。
本研究纳入了95例行根治性或部分肾切除术的RCC患者。将同一患者的正常肾组织样本作为对照组。对RCC患者的石蜡块进行免疫组织化学研究。对表达情况进行半定量分析。确定肿瘤分级和分期与这些表达的关系。结果采用Mann Whitney U检验和Kruskal Wallis检验进行计算。
本研究中有55例男性患者（57.8%）和40例女性患者（42.2%）。患者的年龄和性别与抗体表达无关（p>0.05）。癌组织中ALDH-1和巢蛋白的表达高于正常组织（p<0.05）。肾癌细胞组织中巢蛋白的表达与肿瘤分期呈负相关（p<0.05），但与Fuhrman分级无关。ALDH-1的表达与肿瘤分级相关（p<0.05），但与肿瘤分期无关（p>0.05）。
ALDH-1和巢蛋白的表达在RCC病理生理过程中发挥作用。有必要针对RCC的干细胞标志物开展更多研究，纳入更多患者，且在未来研究中应包括淋巴结转移、远处器官转移和生存率等内容。
