[甲基强的松龙对大鼠创伤性脊髓损伤后神经行为及脑源性神经营养因子和N-甲基-D-天冬氨酸受体表达的影响]

[Effect of methylprednisolone on neurological behavior and the BDNF and NMDA receptor expression after traumatic spinal cord injury in rats].

作者信息

Yang Jun, Xu Zheng-Guang, Rong Rong, Lü Zhi-Ping, Sun Yan-Hua, Zhao Ya, Li Xiao-Song, Liu Jiang-Hua, Dan Qi-Qin, Zhao Nan

机构信息

Department of Bone Injury, Yuxi Municipal Hospital of Traditional Chinese Medicine, Yuxi 653100, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2012 Mar;43(2):245-9.

PMID:22650041

Abstract

OBJECTIVE

To study the effect of methylprednisolone on spinal cord injury rats' neural behavior and the expression of brain derived neurotrophic factor (BDNF) and N-methyl-D-aspartic acid (NMDA).

METHODS

To establish rat model of spinal cord injury (SCI), the rats were randomly divided into sham operation group, SCI group and methylprednisolone group (n = 16 in each group). Eight rats were used for the behavioral assessment and BDNF measurement,the other eight animals was for the NMDA receptor test in each group. Within 8 h spinal cord contusion, methylprednisolone (50 mg/kg) was injected for methylprednisolone group, then after that the intramuscular injection of methylprednisolone was per day reduction in 10 mg/kg, till 5 days. By using immunohistochemical staining, the distribution of BDNF in the spinal cord and positive cell localization was observed and the number of positive cells were counted. The NMDA receptor affinity (Kd) and maximum binding amount (Bmax) were measured with [3H] MK-801 radioligand method, and the rat hind limb functional was also evaluated with BBB score analysis.

RESULTS

Both the number of BDNF positive cells and the BBB score in methylprednisolone group was significant higher than that of SCI group; While increased receptor affinity (Kd) and decreased Bmax for NMDA receptor in methylprednisolone group was seen less than in SCI group (P < 0.05).

CONCLUSION

Methylprednisolone can improve the function of rat hind limb, increase BDNF level and decreased NMDA receptor expression after spinal cord injury.

摘要

目的

研究甲基强的松龙对脊髓损伤大鼠神经行为及脑源性神经营养因子（BDNF）和N-甲基-D-天冬氨酸（NMDA）表达的影响。

方法

建立大鼠脊髓损伤（SCI）模型，将大鼠随机分为假手术组、SCI组和甲基强的松龙组（每组n = 16）。每组8只大鼠用于行为学评估和BDNF检测，另外8只动物用于NMDA受体检测。在脊髓挫伤后8小时内，甲基强的松龙组注射甲基强的松龙（50 mg/kg），之后每天肌肉注射甲基强的松龙，剂量以10 mg/kg递减，直至5天。采用免疫组织化学染色法，观察脊髓中BDNF的分布及阳性细胞定位，并计数阳性细胞数量。用[3H]MK-801放射配体法测定NMDA受体亲和力（Kd）和最大结合量（Bmax），并用BBB评分分析法评估大鼠后肢功能。