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点燃大鼠所生孩子海马中 PSA-NCAM 表达受匹罗卡品诱导癫痫发作的影响。

The effect of PTZ-induced epileptic seizures on hippocampal expression of PSA-NCAM in offspring born to kindled rats.

机构信息

Department of Anatomy and Cell Biology, Mashhad, Iran.

出版信息

J Biomed Sci. 2012 May 31;19(1):56. doi: 10.1186/1423-0127-19-56.

DOI:10.1186/1423-0127-19-56
PMID:22651102
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3586948/
Abstract

BACKGROUND

Maternal epileptic seizures during pregnancy can affect the hippocampal neurons in the offspring. The polysialylated neural cell adhesion molecule (PSA-NCAM), which is expressed in the developing central nervous system, may play important roles in neuronal migration, synaptogenesis, and axonal outgrowth. This study was designed to assess the effects of kindling either with or without maternal seizures on hippocampal PSA-NCAM expression in rat offspring.

METHODS

Forty timed-pregnant Wistar rats were divided into four groups: A) Kind+/Seiz+, pregnant kindled (induced two weeks prior to pregnancy) rats that received repeated intraperitoneal (i.p.) pentylenetetrazol, PTZ injections on gestational days (GD) 14-19; B) Kind-/Seiz+, pregnant non-kindled rats that received PTZ injections on GD14-GD19; C) Kind+/Seiz-, pregnant kindled rats that did not receive any PTZ injections; and D) Kind-/Seiz-, the sham controls. Following birth, the pups were sacrificed on PD1 and PD14, and PSA-NCAM expression and localization in neonates' hippocampi were analyzed by Western blots and immunohistochemistry.

RESULTS

Our data show a significant down regulation of hippocampal PSA-NCAM expression in the offspring of Kind+/Seiz+ (p = 0.001) and Kind-/Seiz+ (p = 0.001) groups compared to the sham control group. The PSA-NCAM immunoreactivity was markedly decreased in all parts of the hippocampus, especially in the CA3 region, in Kind+/Seiz+ (p = 0.007) and Kind-/Seiz+ (p = 0.007) group's newborns on both PD1 and 14.

CONCLUSION

Our findings demonstrate that maternal seizures but not kindling influence the expression of PSA-NCAM in the offspring's hippocampi, which may be considered as a factor for learning/memory and cognitive impairments reported in children born to epileptic mothers.

摘要

背景

孕妇癫痫发作可影响后代海马神经元。在中枢神经系统发育过程中表达的多聚唾液酸神经细胞黏附分子（PSA-NCAM）可能在神经元迁移、突触形成和轴突生长中发挥重要作用。本研究旨在评估点燃合并或不合并母体癫痫对大鼠后代海马 PSA-NCAM 表达的影响。

方法

40 只定时怀孕的 Wistar 大鼠分为 4 组：A）Kind+/Seiz+，怀孕点燃（怀孕前两周诱导）大鼠，在妊娠第 14-19 天接受重复腹腔内（i.p.）戊四氮（PTZ）注射；B）Kind-/Seiz+，怀孕未点燃大鼠，在 GD14-GD19 接受 PTZ 注射；C）Kind+/Seiz-，怀孕未点燃大鼠，未接受任何 PTZ 注射；D）Kind-/Seiz-，假手术对照。出生后，在 PD1 和 PD14 处死幼仔，并通过 Western blot 和免疫组织化学分析新生仔海马 PSA-NCAM 的表达和定位。

结果

我们的数据显示，Kind+/Seiz+（p=0.001）和 Kind-/Seiz+（p=0.001）组后代海马 PSA-NCAM 表达显著下调，与假手术对照组相比。Kind+/Seiz+（p=0.007）和 Kind-/Seiz+（p=0.007）组新生仔海马的所有部位，尤其是 CA3 区，PSA-NCAM 免疫反应明显减弱。

结论

我们的发现表明，母体癫痫而非点燃会影响后代海马中 PSA-NCAM 的表达，这可能是癫痫母亲所生孩子报告的学习/记忆和认知障碍的一个因素。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29ca/3586948/50b6045f90c1/1423-0127-19-56-7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29ca/3586948/2d3393914e67/1423-0127-19-56-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29ca/3586948/8cd2ea90a5a6/1423-0127-19-56-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29ca/3586948/1d0316ada0b9/1423-0127-19-56-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29ca/3586948/4847d291bd84/1423-0127-19-56-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29ca/3586948/5a8e3356bc27/1423-0127-19-56-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29ca/3586948/a67a462d577f/1423-0127-19-56-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29ca/3586948/50b6045f90c1/1423-0127-19-56-7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29ca/3586948/2d3393914e67/1423-0127-19-56-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29ca/3586948/8cd2ea90a5a6/1423-0127-19-56-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29ca/3586948/1d0316ada0b9/1423-0127-19-56-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29ca/3586948/4847d291bd84/1423-0127-19-56-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29ca/3586948/5a8e3356bc27/1423-0127-19-56-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29ca/3586948/a67a462d577f/1423-0127-19-56-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29ca/3586948/50b6045f90c1/1423-0127-19-56-7.jpg

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