用于放射受体分析和放射免疫分析的生物活性放射性碘化胆囊收缩素的制备。
Preparation of biologically active radioiodinated cholecystokinin for radioreceptor assay and radioimmunoassay.
作者信息
Sankaran H, Deveney C W, Goldfine I D, Williams J A
出版信息
J Biol Chem. 1979 Oct 10;254(19):9349-51.
A modified method is described for the preparation of stable, high specific activity radioiodinated cholecystokinin (CCK) by its conjugation to 125I-Bolton Hunter reagent (125I-BH). Purified radioiodinated CCK (125I-BH-CCK) stimulated amylase release from isolate rat pancreatic acini with a potency identical to that of native CCK. Further, 125I-BH-CCK was highly immunoreactive and reacted with antisera directed toward both the NH2- and COOH-terminal portions of the CCK molecule. Finally, 125I-BH-CCK bound to specific receptor sites on isolated pancreatic acini.
描述了一种改进的方法，通过将胆囊收缩素（CCK）与125I-博尔顿·亨特试剂（125I-BH）偶联来制备稳定的、高比活性的放射性碘化CCK。纯化的放射性碘化CCK（125I-BH-CCK）刺激分离的大鼠胰腺腺泡释放淀粉酶，其效力与天然CCK相同。此外，125I-BH-CCK具有高度免疫反应性，能与针对CCK分子氨基末端和羧基末端部分的抗血清发生反应。最后，125I-BH-CCK与分离的胰腺腺泡上的特异性受体位点结合。
Zotero 插件