Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Seizure. 2012 Sep;21(7):508-11. doi: 10.1016/j.seizure.2012.05.003. Epub 2012 May 29.
Our previous study using proteomic analysis showed that superoxide dismutase 1 (SOD1) was significantly decreased in cerebrospinal fluid (CSF) of patients with epilepsy. However, the relevance of CSF-SOD1 alterations for the pathophysiology of epilepsy is currently unknown. The present study was intended to add to our understanding of this issue by measuring SOD1 levels in the CSF of patients with resistant epilepsy and non-resistant epilepsy.
A total of 52 patients with epilepsy were recruited. 29 were non-resistant, 23 drug-resistant. 20 individuals with no evidence of any neurological diseases were used as control. The concentration of CSF and serum SOD1 was measured by enzyme-linked immunosorbent assay.
The concentration of CSF-SOD1 was decreased in both the drug-resistant (0.13 ± 0.12 ng/ml) and the non-resistant epilepsy subgroups (0.29 ± 0.23 ng/ml) compared to the control group (0.40 ± 0.35 ng/ml). SOD1 was significantly lower in the drug-resistant than the non-resistant epilepsy subgroup (P<0.05).
SOD1 levels are decreased in the CSF of patients with epilepsy, especially of patients with intractable epilepsy. Low CSF-SOD1 levels may be a predictor of antiepileptic drug resistance in patients with epilepsy.
我们之前的蛋白质组学分析研究表明，癫痫患者脑脊液（CSF）中的超氧化物歧化酶 1（SOD1）显著降低。然而，CSF-SOD1 改变与癫痫病理生理学的相关性目前尚不清楚。本研究旨在通过测量耐药性癫痫和非耐药性癫痫患者 CSF 中的 SOD1 水平，进一步了解这一问题。
共招募了 52 名癫痫患者。其中 29 名是非耐药性的，23 名是耐药性的。20 名无任何神经疾病证据的个体作为对照组。采用酶联免疫吸附试验测量 CSF 和血清 SOD1 的浓度。
与对照组（0.40 ± 0.35 ng/ml）相比，耐药性癫痫组（0.13 ± 0.12 ng/ml）和非耐药性癫痫组（0.29 ± 0.23 ng/ml）的 CSF-SOD1 浓度均降低。耐药性癫痫组的 SOD1 明显低于非耐药性癫痫组（P<0.05）。
癫痫患者的 CSF 中 SOD1 水平降低，尤其是耐药性癫痫患者。低 CSF-SOD1 水平可能是癫痫患者对抗癫痫药物耐药的预测指标。
