Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
Biomed Mater. 2012 Aug;7(4):045007. doi: 10.1088/1748-6041/7/4/045007. Epub 2012 Jun 1.
Surfaces that preferentially bind human serum albumin (HSA) were generated by grafting albumin-binding linear peptide (LP1) onto silicon surfaces. The research aim was to evaluate the adsorption pattern of proteins and the adhesion of platelets from platelet-poor plasma and platelet-rich plasma, respectively, by albumin-binding surfaces under physiological shear rate (96 and 319 s(-1)) conditions. Bound proteins were quantified using enzyme-linked immunosorbent assays (ELISAs) and two-dimensional gel electrophoresis. A ratio of ∼1000:100:1 of adsorbed HSA, human immunoglobulin (HIgG) and human fibrinogen (HFib) was noted, respectively, on LP1-functionalized surfaces, and a ratio of ∼5:2:1 of the same was noted on control surfaces, as confirmed by ELISAs. The surface-adsorbed von Willebrand factor was undetectable by sensitive ELISAs. The amount of adhered platelets correlated with the ratio of adsorbed HSA/HFib. Platelet morphology was more rounded on LP1-functionalized surfaces when compared to control surfaces. The platelet adhesion response on albumin-binding surfaces can be explained by the reduction in the co-adsorption of other plasma proteins in a surface environment where there is an excess of albumin molecules, coupled with restrictions in the conformational transitions of other surface-adsorbed proteins into hemostatically active forms.
通过将白蛋白结合线性肽 (LP1) 接枝到硅表面上，生成优先结合人血清白蛋白 (HSA) 的表面。研究目的是评估在生理剪切速率（96 和 319 s(-1)) 条件下，白蛋白结合表面对蛋白质的吸附模式以及分别来自血小板减少血浆和富含血小板血浆的血小板的粘附。使用酶联免疫吸附测定 (ELISA) 和二维凝胶电泳定量结合蛋白。在 LP1 功能化表面上，分别观察到吸附的 HSA、人免疫球蛋白 (HIgG) 和人纤维蛋白原 (HFib) 的比率约为 1000:100:1，在对照表面上，分别观察到相同的比率约为 5:2:1，ELISA 证实了这一点。敏感 ELISA 无法检测到表面吸附的血管性血友病因子。粘附的血小板数量与吸附的 HSA/HFib 比率相关。与对照表面相比，LP1 功能化表面上的血小板形态更加圆润。在白蛋白结合表面上的血小板粘附反应可以通过在表面环境中减少其他血浆蛋白的共同吸附来解释，在该表面环境中存在过量的白蛋白分子，同时限制了其他表面吸附蛋白向止血活性形式的构象转变。
