Eshkoor S A, Marashi S J, Ismail P, Rahman S A, Mirinargesi M, Adon M Y, Devan R V
Institute of Gerontology, Universiti Putra Malaysia, Selangor, Malaysia.
Genet Mol Res. 2012 May 21;11(2):1486-96. doi: 10.4238/2012.May.21.5.
We evaluated the possible influence of glutathione S-transferase mu (GSTM1) and glutathione S-transferase theta (GSTT1) genes on genetic damage due to occupational exposure, which contributes to accelerate ageing. This study was conducted on 120 car auto repair workshop workers exposed to occupational hazards and 120 controls without this kind of exposure. The null and non-null genotypes of GSTM1 and GSTT1 genes were determined by multiplex PCR. Micronucleus frequency, Comet tail length and relative telomere length differences between the null and non-null genotypes of the GSTM1 gene were significantly greater in the exposed group. Lack of GSTT1 did not affect the damage biomarkers significantly (P > 0.05), while lack of GSTM1 was associated with greater susceptibility to genomic damage due to occupational exposure. It was concluded that early ageing is under the influence of these genes and the environmental and socio-demographic factors. Duration of working time was significantly associated with micronucleus frequency, Comet tail length and relative telomere length.
我们评估了谷胱甘肽S-转移酶μ（GSTM1）和谷胱甘肽S-转移酶θ（GSTT1）基因对职业暴露所致遗传损伤的可能影响，职业暴露会加速衰老。本研究对120名暴露于职业危害的汽车修理车间工人和120名无此类暴露的对照者进行。通过多重PCR确定GSTM1和GSTT1基因的无效和非无效基因型。暴露组中，GSTM1基因无效和非无效基因型之间的微核频率、彗星尾长和相对端粒长度差异显著更大。缺乏GSTT1对损伤生物标志物无显著影响（P>0.05），而缺乏GSTM1与职业暴露所致基因组损伤的易感性增加有关。得出的结论是，早衰受这些基因以及环境和社会人口因素的影响。工作时间长短与微核频率、彗星尾长和相对端粒长度显著相关。
