Lin Y Q, Xu Y O, Yue Y, Jin S Y, Qu Y, Dong F, Li Y P, Zheng Y C
College of Life Science and Technology, Southwest University for Nationalities, Chengdu, P.R. China.
Genet Mol Res. 2012 Aug 29;11(3):2871-7. doi: 10.4238/2012.May.22.3.
Hypoxia can affect energy metabolism. We examined gene expression and enzyme activity related to mitochondrial energy metabolism, as well as myosin heavy chain (MyHC) types in yaks (Bos grunniens) living at high altitudes. Real-time quantitative PCR assays indicated that the yak has significantly lower levels of carnitine palmitoyltransferase (CPT) mRNA in the biceps femoris and lower levels of uncoupling protein 3 (UCP3) mRNA in both biceps femoris and longissimus dorsi than in Yellow cattle. No significant differences between yak and Yellow cattle were observed in the activities of mitochondrial β-hydroxyacyl-CoA dehydrogenase, isocitrate dehydrogenase and cytochrome oxidase in the same muscles. Semi-quantitative RT-PCR analysis showed that the MyHC 1 mRNA levels in yak biceps femoris was lower than in Yellow cattle. We conclude that the yak has significantly lower mRNA levels of CPT, UCP3, and MyHC 1 in biceps femoris than in Yellow cattle, suggesting that the yak biceps femoris has lower fatty acid oxidation capacity and greater glycolytic metabolic potential.
缺氧会影响能量代谢。我们研究了生活在高海拔地区的牦牛（Bos grunniens）中与线粒体能量代谢相关的基因表达和酶活性，以及肌球蛋白重链（MyHC）类型。实时定量PCR分析表明，牦牛股二头肌中肉碱棕榈酰转移酶（CPT）mRNA水平显著低于黄牛，股二头肌和背最长肌中解偶联蛋白3（UCP3）mRNA水平也低于黄牛。在相同肌肉中，牦牛和黄牛的线粒体β-羟酰基辅酶A脱氢酶、异柠檬酸脱氢酶和细胞色素氧化酶活性未观察到显著差异。半定量RT-PCR分析表明，牦牛股二头肌中MyHC 1 mRNA水平低于黄牛。我们得出结论，牦牛股二头肌中CPT、UCP3和MyHC 1的mRNA水平显著低于黄牛，这表明牦牛股二头肌具有较低的脂肪酸氧化能力和较高的糖酵解代谢潜力。
