Departamento de Biotecnologia-LOT, Escola de Engenharia de Lorena, Universidade de São Paulo, Estrada Municipal do Campinho, PO Box 116, 12602810 Lorena, São Paulo, Brazil.
Appl Biochem Biotechnol. 2012 Aug;167(7):2054-67. doi: 10.1007/s12010-012-9749-x. Epub 2012 Jun 1.
Ten yeast strains were evaluated concerning their capabilities to assimilate biodiesel-derived glycerol in batch cultivation. The influence of glycerol concentration, temperature, pH and yeast extract concentration on biomass production was studied for the yeast selected. Further, the effect of agitation on glycerol utilization by the yeast Hansenula anomala was also studied. The yeast H. anomala CCT 2648 showed the highest biomass yield (0.30 g g(-1)) and productivity (0.19 g L(-1) h(-1)). Citric acid, succinic acid, acetic acid and ethanol were found as the main metabolites produced. The increase of yeast extract concentration from 1 to 3 g L(-1) resulted in high biomass production. The highest biomass concentration (21 g L(-1)), yield (0.45 g g(-1)) and productivity (0.31 g L(-1) h(-1)), as well as ribonucleotide production (13.13 mg g(-1)), were observed at 700 rpm and 0.5 vvm. These results demonstrated that glycerol from biodiesel production process showed to be a feasible substrate for producing biomass and ribonucleotides by yeast species.
评估了 10 株酵母菌株在分批培养中同化生物柴油衍生甘油的能力。研究了所选酵母的甘油浓度、温度、pH 和酵母提取物浓度对生物量生产的影响。此外，还研究了搅拌对异常汉逊酵母利用甘油的影响。酵母异常汉逊酵母 CCT 2648 表现出最高的生物量产量（0.30 g g(-1)）和生产力（0.19 g L(-1) h(-1)）。发现柠檬酸、琥珀酸、乙酸和乙醇是主要的代谢产物。酵母提取物浓度从 1 增加到 3 g L(-1)导致生物量产量增加。在 700 rpm 和 0.5 vvm 下，观察到最高的生物量浓度（21 g L(-1)）、产量（0.45 g g(-1)）和生产力（0.31 g L(-1) h(-1)），以及核苷酸产量（13.13 mg g(-1)）。这些结果表明，生物柴油生产过程中的甘油可以作为生产生物量和核苷酸的酵母的可行底物。
