Department of Anesthesiology and Reanimation, Ankara Training and Research Hospital, Minister of Health, Ankara, Turkey.
Hum Exp Toxicol. 2012 Dec;31(12):1207-13. doi: 10.1177/0960327112446818. Epub 2012 May 31.
In this study, genotoxic activities of four halogenated anesthetics (halothane, isoflurane, sevoflurane and desflurane) were investigated in human peripheral blood lymphocytes (PBLs) and sperm cells in vitro by alkaline comet assay. For this purpose, sperm or lymphocyte suspension was exposed to different concentrations (0.1 mM, 1 mM, 10 mM and 100 mM) of anesthetic agents and 1% dimethyl sulfoxide (DMSO) or phosphate-buffered saline (PBS) as controls. The DNA strand breaks as well as alkali-labile sites were measured as percentage tail intensity with comet assay. The results of this study demonstrate that all analyzed drugs were capable of inducing DNA damage on PBLs in a dose-dependent manner in vitro. However, the results in sperm cells were slightly different since we did not observe any genotoxic effect for desflurane in any of the exposure doses, and the genotoxic effect of halothane was not dose dependent. This experimental study points out to the presence of DNA damage after exposure to halogenated anesthetics in both PBLs and sperm cells, although this effect seems to be higher in PBLs.
本研究通过碱性彗星试验，研究了四种卤代麻醉剂（氟烷、异氟烷、七氟烷和地氟烷）在人外周血淋巴细胞（PBLs）和精子细胞中的遗传毒性活性。为此，将精子或淋巴细胞悬浮液暴露于不同浓度（0.1mM、1mM、10mM 和 100mM）的麻醉剂和 1%二甲基亚砜（DMSO）或磷酸盐缓冲盐水（PBS）作为对照。用彗星试验测量 DNA 链断裂和碱不稳定部位的尾部强度百分比。本研究结果表明，所有分析的药物都能够以剂量依赖的方式在体外诱导 PBLs 的 DNA 损伤。然而，精子细胞的结果略有不同，因为我们没有观察到地氟烷在任何暴露剂量下都有遗传毒性作用，而且氟烷的遗传毒性作用也不是剂量依赖性的。这项实验研究指出，在 PBLs 和精子细胞中接触卤代麻醉剂后会出现 DNA 损伤，尽管这种效应在 PBLs 中似乎更高。
