乳腺囊肿液对MCF-7乳腺癌细胞中雌激素17-氧化还原酶活性的影响。

Effect of breast cyst fluid on oestrogen 17-oxidoreductase activity in MCF-7 breast cancer cells.

作者信息

Lai L C, Coldham N G, Islam S, Reed M J, Ghilchik M W, James V H

机构信息

Department of Chemical Pathology, St. Mary's Hospital Medical School, London, U.K.

出版信息

Cancer Lett. 1990 Dec 3;55(2):165-9. doi: 10.1016/0304-3835(90)90028-v.

DOI:10.1016/0304-3835(90)90028-v

PMID:2265417

Abstract

Breast cyst fluid (BCF) was found to stimulate oestrogen 17-oxidoreductase activity in the reductive direction, i.e., conversion of oestrone (E1) to oestradiol (E2), in MCF-7 breast cancer cells. Dialysis of BCF revealed that this property of BCF was present in both dialysed BCF and dialysate, implying that both high and low mol. wt. substances were responsible for stimulating E1 to E2 conversion. Gel filtration of dialysed BCF revealed that the high mol. wt. substances responsible for the stimulation of E1 to E2 conversion had mol. wts. of approximately 11 kD and 68 kD. This property of BCF would serve to increase the concentration of E2, a steroid which may play a role in mammary carcinogenesis.

摘要

研究发现，乳腺癌囊液（BCF）可刺激MCF-7乳腺癌细胞中雌激素17-氧化还原酶的还原活性，即将雌酮（E1）转化为雌二醇（E2）。对BCF进行透析后发现，透析后的BCF和透析液均具有这种特性，这表明高分子量和低分子量物质均参与刺激E1向E2的转化。对透析后的BCF进行凝胶过滤分析，结果显示，负责刺激E1向E2转化的高分子量物质的分子量约为11kD和68kD。BCF的这一特性有助于提高E2的浓度，而E2这种类固醇激素可能在乳腺癌发生过程中发挥作用。