半乳糖凝集素-10在阿司匹林敏感性呼吸道疾病患者的鼻腔灌洗液中释放。
Galectin-10 is released in the nasal lavage fluid of patients with aspirin-sensitive respiratory disease.
作者信息
Negrete-Garcia Ma Cristina, Jiménez-Torres Carla Yoneli, Alvarado-Vásquez Noe, Montes-Vizuet A Rosalía, Velázquez-Rodriguez J R, Jimenez-Martinez M Carmen, Teran-Juárez Luis Manuel
机构信息
Department of Immunogenetics and Allergy, Instituto Nacional de Enfermedades Respiratorias, Calzada Tlalpan 4502, 14080 Mexico, DF, Mexico.
出版信息
ScientificWorldJournal. 2012;2012:474020. doi: 10.1100/2012/474020. Epub 2012 Apr 30.
The aim of this work was to determine the presence of galectin-10 in nasal lavage fluid (NLF) of patients with aspirin-sensitive respiratory disease (ASRD) before and after challenge with L-ASA (aspirin) by ELISA. Fifteen ASRD patients, ten aspirin-tolerant asthmatics (ATA), and fifteen healthy controls (HC) were studied. The baseline presence of Galectin-10 in PBMC was determined using real time PCR. Galectin-10 was evaluated in tissue of nasal polyps by western blot. Our results showed a lower expression in PBMC of ASRD patients than in ATA and healthy controls. However, a higher concentration of galectin-10 in NLF was found in ASRD patients before and after L-ASA challenge; western blot confirmed a high expression of galectin-10 in tissue from nasal polyps obtained from ASRD patients. Our results suggest a probable role of galectin-10 in the inflammatory response observed in ASRD patients; however, confirmatory studies are needed.
这项工作的目的是通过酶联免疫吸附测定（ELISA）来确定阿司匹林敏感呼吸道疾病（ASRD）患者在L-ASA（阿司匹林）激发前后鼻灌洗液（NLF）中半乳糖凝集素-10的存在情况。研究了15例ASRD患者、10例阿司匹林耐受哮喘患者（ATA）和15例健康对照者（HC）。使用实时聚合酶链反应（PCR）测定外周血单核细胞（PBMC）中半乳糖凝集素-10的基线存在情况。通过蛋白质免疫印迹法（western blot）评估鼻息肉组织中的半乳糖凝集素-10。我们的结果显示，ASRD患者PBMC中的表达低于ATA患者和健康对照者。然而，在L-ASA激发前后，ASRD患者的NLF中半乳糖凝集素-10浓度较高；蛋白质免疫印迹法证实，从ASRD患者获得的鼻息肉组织中半乳糖凝集素-10表达较高。我们的结果表明，半乳糖凝集素-10在ASRD患者观察到的炎症反应中可能起作用；然而，还需要进行验证性研究。
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