对一种与神经元死亡同时出现在烟草天蛾神经系统中的蛋白质的表征。

Characterization of a protein that appears in the nervous system of the moth Manduca sexta coincident with neuronal death.

作者信息

Montemayor M E, Fahrbach S E, Giometti C S, Roy E J

机构信息

Department of Entomology, University of Illinois, Urbana-Champaign 61820.

出版信息

FEBS Lett. 1990 Dec 10;276(1-2):219-22. doi: 10.1016/0014-5793(90)80546-u.

DOI:10.1016/0014-5793(90)80546-u

PMID:2265704

Abstract

Two-dimensional gel electrophoresis was used to locate potential neuronal death-related proteins in the moth Manduca sexta. Protein patterns of ganglia of pharate adult moths (taken prior to adult ecdysis) compared with protein patterns of one-day-old adults revealed reproducible changes in protein patterns. An acidic protein of approximately 40,000 Da was present in all samples from adult moths undergoing neuronal death and essentially absent from pharate adult samples.

摘要

利用二维凝胶电泳定位烟草天蛾中潜在的与神经元死亡相关的蛋白质。将即将羽化的成年蛾（在成虫蜕皮前采集）神经节的蛋白质图谱与一日龄成虫的蛋白质图谱进行比较，结果显示蛋白质图谱发生了可重复的变化。在所有经历神经元死亡的成年蛾样本中都存在一种分子量约为40000道尔顿的酸性蛋白质，而在即将羽化的成年蛾样本中基本不存在。