Barneo L, Esteban M M, Garcia-Pravia C, Diaz F, Marin B
Department of Functional Biology-Physiology, University of Oviedo, Spain.
Diabete Metab. 1990 Jul-Aug;16(4):284-9.
The purpose of our study was to determine if streptozotocin induced diabetes (SID) in rats produces alterations in hepatic function, as described in poorly controlled diabetic patients, and if islet transplantation (islet-Tx) would subsequently ameliorate this status. Hepatocellular dysfunction was evaluated by the aspartate aminotransferase (SGOT) and the alanine aminotransferase (SGPT) activities in plasma. For the evaluation of cholestasis the plasma alkaline phosphatase (APase) activity was used. These determinations were performed in normal, SID, SID with Islet-Tx, and SID Wistar rats with sham-Tx. Also, glucose was measured in plasma samples, as well as histological studies of the liver were performed. More than 1,000 isogeneic islets (islet-Tx group) or non viable insular tissue (sham-Tx group) were transplanted via mesenteric ileal vein three weeks after SID. The results showed that SID in rats produces alterations in the hepatic function as well as in the structure of the hepatocytes, and the normalization of carbohydrate metabolism by islet transplantation restores normal hepatic function and morphology.
我们研究的目的是确定大鼠中链脲佐菌素诱导的糖尿病（SID）是否会像控制不佳的糖尿病患者那样引起肝功能改变，以及胰岛移植（islet-Tx）是否随后会改善这种状况。通过测定血浆中天冬氨酸转氨酶（SGOT）和丙氨酸转氨酶（SGPT）的活性来评估肝细胞功能障碍。为评估胆汁淤积，使用血浆碱性磷酸酶（APase）活性。这些测定在正常大鼠、SID大鼠、接受胰岛移植的SID大鼠以及接受假手术的SID Wistar大鼠中进行。此外，还测定了血浆样本中的葡萄糖，并对肝脏进行了组织学研究。在SID发生三周后，通过肠系膜回肠静脉移植了1000多个同基因胰岛（胰岛移植组）或无活力的胰岛组织（假手术组）。结果表明，大鼠中的SID会引起肝功能以及肝细胞结构的改变，而通过胰岛移植使碳水化合物代谢正常化可恢复正常的肝功能和形态。
