Dumoulin J C, Evers J L, Offermans J P, Bras M, Pieters M H, Geraedts J P
Department of Obstetrics and Gynaecology, Academic Hospital Maastricht, University of Limburg, The Netherlands.
Gynecol Obstet Invest. 1990;30(3):165-8. doi: 10.1159/000293254.
In two separate prospective randomized series of 38 and 16 attempts of human in vitro fertilization (IVF), respectively, the fertilizing abilities of human spermatozoa capacitated in hyperosmotic media and in normosmotic medium were compared. Oocytes from each IVF attempt were divided into two equal groups and inseminated with spermatozoa incubated for 2 h in medium with an osmolality of 285 mosm/kg or in hyperosmotic medium of 315 (first experiment) or 345 mosm/kg (second experiment). The fertilizing capacity of the spermatozoa was not affected by incubation in medium of 315 mosm/kg as compared to spermatozoa incubated in medium of 285 mosm/kg. However, the fertilizing capacity of the spermatozoa incubated in medium of 345 mosm/kg was significantly reduced. The present study indicates that there is no advantage in the use of hyperosmotic media of 315 or 345 mosm/kg for capacitation of spermatozoa in human IVF.
在两个分别有38次和16次人类体外受精（IVF）尝试的前瞻性随机系列研究中，分别比较了在高渗介质和等渗介质中获能的人类精子的受精能力。每次IVF尝试获得的卵母细胞被分成两组，分别与在渗透压为285毫渗摩尔/千克的介质中孵育2小时的精子，以及在渗透压为315毫渗摩尔/千克（第一个实验）或345毫渗摩尔/千克（第二个实验）的高渗介质中孵育2小时的精子进行受精。与在285毫渗摩尔/千克介质中孵育的精子相比，在315毫渗摩尔/千克介质中孵育的精子的受精能力没有受到影响。然而，在345毫渗摩尔/千克介质中孵育的精子的受精能力显著降低。本研究表明，在人类IVF中，使用315或345毫渗摩尔/千克的高渗介质对精子进行获能没有优势。
