Department of Forensic Medicine and Toxicology, School of Medicine, National and Kapodistrian University of Athens, Greece.
J Pharm Biomed Anal. 2012 Nov;70:557-62. doi: 10.1016/j.jpba.2012.05.007. Epub 2012 May 15.
Antidepressant drugs are widely used for the treatment of depression and other psychiatric disorders and as a result they are involved in numerous clinical and forensic cases. The aim of this study was the development, optimization and validation of a simple, specific and sensitive GC/MS method for the simultaneous determination of 11 antidepressant drugs and 4 of their metabolites (amitriptyline, citalopram, clomipramine, fluoxetine, fluvoxamine, maprotiline, desmethyl-maprotiline, mirtazapine, desmethyl-mirtazapine, nortriptyline, paroxetine, sertraline, desmethyl-sertraline, venlafaxine and desmethyl-venlafaxine) in whole blood. The combination of solid-phase extraction with derivatization using heptafluorobutyric anhydride efficiently reduced matrix effect and improved sensitivity of the method. In this assay, protriptyline was used as internal standard. Absolute recovery values for all analytes were ranged from 79.2 to 102.6%. LODs and LOQs were found to be between 0.30-1.50 μg/L and 1.00-5.00 μg/L, respectively. The calibration curves were linear (R(2)≥0.990) within the range of 5.00-1000 μg/L for all analytes. Accuracy expressed as the % E(r) was found to be between -12.3 and 12.2%. Precision expressed as the % RSD was found to be less than 11.7% for all antidepressants. The developed method proved to be suitable for routine work and it was used to successfully analyze more than 2500 clinical and forensic blood samples.
抗抑郁药被广泛用于治疗抑郁症和其他精神疾病，因此它们涉及到许多临床和法医案例。本研究的目的是开发、优化和验证一种简单、特异和灵敏的 GC/MS 方法，用于同时测定 11 种抗抑郁药及其 4 种代谢物（阿米替林、西酞普兰、氯米帕明、氟西汀、氟伏沙明、马普替林、去甲马普替林、米氮平、去甲米氮平、去甲替林、帕罗西汀、舍曲林、去甲舍曲林、文拉法辛和去甲文拉法辛）在全血中的浓度。固相萃取与七氟丁酸衍生化相结合，有效地降低了基质效应，提高了方法的灵敏度。在该测定方法中，普罗替林被用作内标。所有分析物的绝对回收率在 79.2%至 102.6%之间。LOD 和 LOQ 分别为 0.30-1.50 μg/L 和 1.00-5.00 μg/L。所有分析物的校准曲线在 5.00-1000 μg/L 范围内均呈线性（R(2)≥0.990）。准确度以 % E(r)表示，在-12.3%至 12.2%之间。精密度以 % RSD 表示，所有抗抑郁药均小于 11.7%。所建立的方法证明适用于常规工作，并成功分析了 2500 多个临床和法医血液样本。
