Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht Str. 74, 20359 Hamburg, Germany.
Vaccine. 2012 Jul 13;30(33):4971-6. doi: 10.1016/j.vaccine.2012.05.046. Epub 2012 May 30.
It is estimated that 30-100 million people are infected with the pathogenic nematode Strongyloides stercoralis worldwide but parasite control is still based on anti-helminthic treatment. To develop protective vaccination strategies, we use the murine model of Strongyloides ratti infection. We have shown recently that vaccination with alum-precipitated, but not with native or CFA-emulsified S. ratti heat shock protein 60 (srHSP60) conferred protection to challenge infection. Here we describe the generation of a monoclonal IgM specific for srHSP60. Anti-srHSP60 detected human and srHSP60 and stained S. ratti infective larvae in vitro. Passive immunization of mice with monoclonal anti-srHSP60 IgM led to reduced numbers of migrating larvae in lung and head, reduced numbers of parasitic adults in the small intestine and reduced larval output upon S. ratti challenge infection. Taken together, our findings highlight the relevance of srHSP60 as vaccine candidate for the induction of antibody-mediated protection against Strongyloides infection.
据估计，全世界有 3000 万至 1 亿人感染了致病线虫旋毛虫，但寄生虫控制仍然基于驱虫治疗。为了开发保护性疫苗接种策略，我们使用了鼠类旋毛虫感染模型。我们最近表明，用明矾沉淀的疫苗接种，而不是用天然或 CFA 乳化的旋毛虫热休克蛋白 60（srHSP60）进行疫苗接种，可以对挑战感染提供保护。在这里，我们描述了生成针对 srHSP60 的单克隆 IgM 的方法。抗 srHSP60 检测到人和 srHSP60，并在体外染色旋毛虫感染性幼虫。用单克隆抗 srHSP60 IgM 对小鼠进行被动免疫，导致肺和头部迁移幼虫数量减少，小肠寄生成虫数量减少，旋毛虫挑战感染后的幼虫产量减少。总之，我们的研究结果强调了 srHSP60 作为疫苗候选物在诱导针对旋毛虫感染的抗体介导保护中的相关性。
