Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke (QC), Canada J1K 2R1.
Immunol Lett. 2012 Aug 30;146(1-2):70-3. doi: 10.1016/j.imlet.2012.05.002. Epub 2012 May 29.
Prostaglandin E(2) (PGE(2)) induces the expression of C-C chemokine receptor type 7 (CCR7) on human monocytes, thereby enabling their subsequent migration in response to CCL19 and CCL21, the natural ligands for CCR7. To date, important mediators of PGE(2)-mediated monocyte migration remain unknown. In this study, we explored the role of mitogen-activated protein kinases and the RhoA/Rho-associated protein kinase (ROCK) pathway in CCR7-dependent monocyte migration in the presence of PGE(2). Our results indicate that CCL19 binding to CCR7 promotes the activation of p38, extracellular signal-regulated kinase 1/2, and c-Jun N-terminal kinase and leads to monocyte migration. Moreover, the RhoA/ROCK pathway was essential for PGE(2)-mediated CCR7-dependent monocyte migration.
前列腺素 E(2) (PGE(2)) 诱导人单核细胞表达 C-C 趋化因子受体 7 (CCR7)，从而使其能够响应 CCR7 的天然配体 CCL19 和 CCL21 进行随后的迁移。迄今为止，PGE(2) 介导的单核细胞迁移的重要介质仍不清楚。在这项研究中，我们探讨了丝裂原活化蛋白激酶和 RhoA/Rho 相关蛋白激酶 (ROCK) 通路在 PGE(2) 存在下 CCR7 依赖性单核细胞迁移中的作用。我们的结果表明，CCL19 与 CCR7 的结合促进了 p38、细胞外信号调节激酶 1/2 和 c-Jun N-末端激酶的激活，并导致单核细胞迁移。此外，RhoA/ROCK 通路对于 PGE(2) 介导的 CCR7 依赖性单核细胞迁移是必不可少的。
