Institut Pasteur, Department of Microbiology, Unité Biologie Moléculaire du Gène chez les Extrêmophiles, 75015 Paris, France.
J Virol Methods. 2012 Oct;185(1):149-51. doi: 10.1016/j.jviromet.2012.05.017. Epub 2012 May 29.
Determining the nature of the viral genome is one of the first steps in characterization of any new virus. However, in the case of viruses with a single-stranded genome, it is not always simple to identify its orientation. In this study, a rapid, sensitive and simple PCR-based method is described to identify the strand orientation of single-stranded viral genomes. This method has been tested on the single-stranded DNA viruses, M13 and phiX174.
确定病毒基因组的性质是对任何新病毒进行特征描述的第一步。然而，对于具有单链基因组的病毒，确定其方向并不总是那么简单。本研究描述了一种快速、灵敏、简单的基于 PCR 的方法，用于鉴定单链病毒基因组的链方向。该方法已在单链 DNA 病毒 M13 和 phiX174 上进行了测试。
