Department of Pathology, National Defense Medical Center, Division of Clinical Pathology, Tri-Service General Hospital, Taipei, Taiwan, ROC.
Clin Biochem. 2012 Nov;45(16-17):1471-8. doi: 10.1016/j.clinbiochem.2012.05.023. Epub 2012 May 31.
The aim was to evaluate the use of combination of SBT (sequence based typing) and HARP (heterozygous ambiguity resolving primer) in HLA typing to acquire high resolution typing results.
167 DNA samples were analyzed by SBT. The web site HARPs Finder provided by Conexio Genomics, the developer of HARPs (http://www.harpsfinder.conexio-genomics.com/index.html) was then used to search for appropriate HARPs.
HARPs can resolve 95% of ambiguities for locus A; 86% for B and 60% for DRB1 locus. However, there are still limitations. Practically PCR products of un-separated alleles are used as templates for sequencing by HARP; sometimes, it is still impossible to get unambiguous typing.
We outlined the advantages and disadvantages of SBT/HARP. A list of HARPs for choice to resolve ambiguity of SBT in Taiwanese population is concluded.
评估 SBT（基于序列的分型）和 HARP（杂合性歧义解决引物）联合应用于 HLA 分型以获得高分辨率分型结果。
对 167 个 DNA 样本进行 SBT 分析。然后使用 Conexio Genomics 开发的 HARPs（http://www.harpsfinder.conexio-genomics.com/index.html）的 HARPs Finder 网站搜索合适的 HARPs。
HARPs 可解决 A 位点 95%的歧义；B 位点 86%；DRB1 位点 60%。然而，仍存在局限性。实际上，未分离等位基因的 PCR 产物被用作 HARP 测序的模板；有时，仍然无法获得明确的分型。
我们概述了 SBT/HARP 的优缺点。总结了用于解决台湾人群 SBT 歧义的 HARPs 选择列表。
