Department of Glycotechnology, Center for Advanced Medical Research, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan.
Biochem Biophys Res Commun. 2012 Jun 29;423(2):344-9. doi: 10.1016/j.bbrc.2012.05.127. Epub 2012 May 31.
Hyaluronan and chondroitin are glycosaminoglycans well-known as components of pharmaceutical agents and health foods. From these attractive molecules, using transglycosylation reaction of testicular hyaluronidase, we synthesized hybrid neo-oligosaccharides not found in nature. We also found a new site between the chondroitin disaccharide unit and hyaluronan disaccharide unit recognized by a hyaluronan lyase specific to hyaluronan using these hybrid oligosaccharides as substrates. We hope that these hybrid oligosaccharides will help to elucidate the involvement of hyaluronan, chondroitin, and chondroitin sulfates in the mechanisms of cell functions and diseases, based on the structures of these glycosaminoglycans.
透明质酸和软骨素是众所周知的糖胺聚糖，是药物制剂和保健品的成分。从这些有吸引力的分子中，我们利用睾丸透明质酸酶的转糖苷反应，合成了自然界中不存在的混合新寡糖。我们还发现了一个新的位点，它位于透明质酸裂解酶特异性识别的透明质酸二糖单元和软骨素二糖单元之间，该酶的底物是这些混合寡糖。我们希望这些混合寡糖将有助于阐明透明质酸、软骨素和硫酸软骨素在细胞功能和疾病机制中的作用，这是基于这些糖胺聚糖的结构。
