Newsome D A, Dobard E P, Liles M R, Oliver P D
Sensory and Electrophysiology Research Unit, Touro Infirmary, New Orleans, LA 70115.
Invest Ophthalmol Vis Sci. 1990 Dec;31(12):2508-13.
Superoxide dismutase (SOD) molecules occur in all cells exposed to an oxygen-containing environment, including retinal pigment epithelial (RPE) cells. Previous studies of nonhuman RPE have either probed specifically for copper-zinc-containing SOD (CuZn-SOD) or have not distinguished between CuZn-SOD and the SOD molecule that contains manganese (Mn-SOD). The authors used specific enzymatic assays and immunologic probes, both in vivo and in vitro, to show that human RPE cells contain both CuZn-SOD and Mn-SOD. The CuZn-SOD had a diffuse cytosolic distribution, whereas the Mn form was located primarily in the mitochondria. The role of SODs in protecting the chorioretinal complex against oxidative damage and with regard to aging processes is not well understood and warrants further investigation, and the two cellular forms of SOD should be considered in future studies.
超氧化物歧化酶（SOD）分子存在于所有暴露于含氧环境的细胞中，包括视网膜色素上皮（RPE）细胞。先前对非人类RPE的研究要么专门探测含铜锌的SOD（CuZn-SOD），要么没有区分CuZn-SOD和含锰的SOD分子（Mn-SOD）。作者在体内和体外使用了特定的酶分析和免疫探针，以表明人类RPE细胞同时含有CuZn-SOD和Mn-SOD。CuZn-SOD在细胞质中呈弥散分布，而锰形式主要位于线粒体中。SOD在保护脉络膜视网膜复合体免受氧化损伤以及在衰老过程中的作用尚未得到充分了解，值得进一步研究，并且在未来的研究中应考虑SOD的两种细胞形式。
