Nückel Holger, Castelli Erick C, Moreau Philippe, Ochsenfarth Crista, Horn Peter A, Rebmann Vera
Department of Haematology and Institute of Pharmacogenetics, University Hospital Essen, Essen, Germany.
Methods Mol Biol. 2012;882:123-42. doi: 10.1007/978-1-61779-842-9_7.
The non-classical human leukocyte antigen (HLA)-G plays a crucial role in the induction of tolerance at the feto-maternal interface as well as in transplantation, cancer, inflammation, and autoimmune diseases. To understand gene regulation and the impact of polymorphic sites on the function, simple and easy feasible approaches are needed for the detection of HLA-G variants in coding and non-coding regions. Here we summarize a set of methods for the identification of variants in the exon 2-4, in the 3' untranslated region and in the gene promoter region of the HLA-G gene.
非经典人类白细胞抗原（HLA）-G在母胎界面的免疫耐受诱导以及移植、癌症、炎症和自身免疫性疾病中发挥着关键作用。为了解基因调控以及多态性位点对功能的影响，需要简单易行的方法来检测编码区和非编码区的HLA-G变体。在此，我们总结了一套用于鉴定HLA-G基因外显子2-4、3'非翻译区和基因启动子区变体的方法。
