Ordóñez David, Moraru Manuela, Gómez-Lozano Natalia, Cisneros Elisa, Vilches Carlos
Inmunogenética-HLA, Hospital Universitario Puerta de Hierro, Majadahonda, Spain.
Methods Mol Biol. 2012;882:415-30. doi: 10.1007/978-1-61779-842-9_24.
The killer-cell immunoglobulin-like receptors (KIR), which enable NK cells to detect allogeneic target cells and abnormalities in the expression of self-HLA molecules, are encoded by genes that display extensive copy number variation. These variations in the KIR genotype are relevant for multiple aspects of human health, including therapy of cancer. PCR with sequence-specific primers (SSP) is simplest and most widely used among techniques for studying KIR genotypes. Here, we present a protocol that details the critical steps of a method for KIR genotyping by PCR-SSP.
杀伤细胞免疫球蛋白样受体（KIR）可使自然杀伤细胞（NK细胞）检测异基因靶细胞以及自身HLA分子表达异常情况，其由显示出广泛拷贝数变异的基因编码。KIR基因型的这些变异与人类健康的多个方面相关，包括癌症治疗。在研究KIR基因型的技术中，序列特异性引物聚合酶链反应（PCR-SSP）是最简单且应用最广泛的。在此，我们展示了一份方案，详细说明了通过PCR-SSP进行KIR基因分型方法的关键步骤。
