Department of Chemistry, Ahmet Keleşoğlu Education Faculty, Konya University, 42099 Konya, Turkey.
Sensors (Basel). 2012;12(4):3916-28. doi: 10.3390/s120403916. Epub 2012 Mar 26.
Electrochemical oxidation of quercetin, as an important biological molecule, has been studied in non-aqueous media using cyclic voltammetry, electrochemical impedance spectroscopy and scanning electron microscopy. To investigate the electrochemical properties of quercetin, an important flavonoid derivative, on a different surface, a new glassy carbon electrode has been developed using dithiooxamide as modifier in non-aqueous media. The surface modification of glassy carbon electrode has been performed within the 0.0 mV and +800 mV potential range with 20 cycles using 1 mM dithioxamide solution in acetonitrile. However, the modification of quercetin to both bare glassy carbon and dithiooxamide modified glassy carbon electrode surface was carried out in a wide +300 mV and +2,800 mV potential range with 10 cycles. Following the modification process, cyclic voltammetry has been used for the surface characterization in aqueous and non-aqueous media whereas electrochemical impedance spectroscopy has been used in aqueous media. Scanning electron microscopy has also been used to support the surface analysis. The obtained data from the characterization and modification studies of dithioxamide modified and quercetin grafted glassy carbon electrode showed that the developed electrode can be used for the quantitative determination of quercetin and antioxidant capacity determination as a chemical sensor electrode.
槲皮素作为一种重要的生物分子，其电化学氧化在非水介质中使用循环伏安法、电化学阻抗谱和扫描电子显微镜进行了研究。为了研究一种重要的类黄酮衍生物槲皮素在不同表面上的电化学性质，在非水介质中使用二硫代乙酰胺作为修饰剂，开发了一种新型的玻碳电极。在 0.0 mV 和 +800 mV 的电位范围内，使用 1 mM 二硫代乙酰胺在乙腈中的溶液进行 20 个循环，对玻碳电极的表面进行修饰。然而，在宽的 +300 mV 和 +2800 mV 电位范围内，用 10 个循环对裸玻碳和二硫代乙酰胺修饰的玻碳电极表面进行了槲皮素的修饰。修饰过程完成后，在水相和非水相介质中使用循环伏安法进行表面表征，而在水相介质中使用电化学阻抗谱法。扫描电子显微镜也被用来支持表面分析。从二硫代乙酰胺修饰和槲皮素接枝玻碳电极的修饰和表征研究中获得的数据表明，所开发的电极可用于槲皮素的定量测定和抗氧化能力测定，作为化学传感器电极。
