College of Life Science and Biotechnology, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, China.
Evid Based Complement Alternat Med. 2012;2012:986427. doi: 10.1155/2012/986427. Epub 2012 May 14.
ZHENG is the key theory in traditional Chinese medicine (TCM) and it is very important to find the molecular pharmacology of traditional Chinese herbal formulae. One ZHENG is related to many diseases and the herbal formulae are aiming to ZHENG. Therefore, many herbal formulae whose effects on a certain disease have been confirmed might also treat other diseases with the same ZHENG. In this study, the microarrays collected from patients with QiXuXueYu ZHENG (Qi-deficiency and Blood-stasis syndrome) before treatment and after being treated with Fuzheng Huayu Capsule were analyzed by a high-throughput gene microarrays-based drug similarity comparison method, which could find the small molecules which had similar effects with Fuzheng Huayu Capsule. Besides getting the results of anti-inflammatory and anti-fibrosis drugs which embody the known effect of Fuzheng Huayu Capsule, many other small molecules were screened out and could reflect other types of effects of this formula in treating QiXuXueYu ZHENG, including anti-hyperglycemic, anti-hyperlipidemic, hyposenstive effect. Then we integrated this information to display the effect of Fuzheng Huayu Capsule and its potential multiple-target molecular pharmacology. Moreover, through using clinical blood-tested data to verify our prediction, Fuzheng Huayu Capsule was proved to have effects on diabetes and dyslipidemia.
“证”是中医理论的关键，寻找中药复方的分子药理学非常重要。一个“证”与许多疾病有关，而中药复方则针对“证”。因此，许多已证实对某种疾病有效的确证中药复方，也可能对具有相同“证”的其他疾病进行治疗。在这项研究中，我们通过高通量基因芯片药物相似性比较方法，分析了治疗气虚血瘀证患者在治疗前后的微阵列，找到了与扶正化瘀胶囊具有相似作用的小分子。除了得到体现扶正化瘀胶囊已知作用的抗炎和抗纤维化药物的结果外，还筛选出许多其他小分子，反映了该方剂治疗气虚血瘀证的其他类型作用，包括降血糖、降血脂、降血压作用。然后，我们整合这些信息，以显示扶正化瘀胶囊的作用及其潜在的多靶点分子药理学。此外，通过使用临床血液测试数据来验证我们的预测，证明扶正化瘀胶囊对糖尿病和血脂异常有作用。
