牙本质和牙釉质在冷冻保存后出现裂纹。
Cracks in dentin and enamel after cryopreservation.
机构信息
School of Dental Medicine, Department of Oral Surgery, Oral Radiology and Oral Medicine, University of Basel, Basel, Switzerland.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol. 2012 Feb;113(2):e5-e10. doi: 10.1016/j.tripleo.2011.06.020. Epub 2012 Jan 20.
OBJECTIVE
The objective of this study was to investigate if cryopreservation of teeth for long-term storage leads to cracks in enamel and dentin.
STUDY DESIGN
Three teeth, which were extracted for orthodontic reasons, were cryopreserved in liquid nitrogen (temperature -196°C) and thawed according to standard protocols after 4 months. Micro computed tomography using synchrotron radiation was performed to detect cracks in the tooth hard tissues.
RESULTS
Cracks were found in the enamel of all teeth, which are associated with forceps application during extraction. Cracks with a width larger than 0.8 μm were not identified in dentin and cementum.
CONCLUSION
Although cryopreservation of teeth according to the standard protocol does not generate cracks more than 0.8 μm wide, the use of forceps can result in prominent cracks.
目的
本研究旨在探讨牙齿的长期冷冻储存是否会导致牙釉质和牙本质出现裂纹。
研究设计
从正畸治疗中拔出的 3 颗牙齿在液氮（温度-196°C）中冷冻保存，并在 4 个月后按照标准方案解冻。使用同步辐射微计算机断层扫描来检测牙齿硬组织中的裂纹。
结果
所有牙齿的牙釉质中都发现了裂纹，这些裂纹与拔牙时使用钳子有关。在牙本质和牙骨质中未发现宽度大于 0.8μm 的裂纹。
结论
尽管按照标准方案对牙齿进行冷冻保存不会产生大于 0.8μm 的裂纹，但钳子的使用可能会导致明显的裂纹。
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