Ambroso Jeffrey, Harris Craig
Safety Assessment, GlaxoSmithKline, Research Triangle Park, NC, USA.
Methods Mol Biol. 2012;889:407-23. doi: 10.1007/978-1-61779-867-2_25.
Histiotrophic nutrition is a process whereby the rodent visceral yolk sac (VYS) internalizes exogenous macromolecules, degrades them, and sends the degradation products to the embryo for use in de novo macromolecular biosynthesis. This process is important for embryonic development during early gestation prior to the formation of the functional placenta. Quantification and visualization of histiotrophic nutrition can be accomplished using fluorescent tracer molecules such as fluorescein isothiocyanate-conjugated albumin (FITC-albumin) that can be visualized using fluorescent microscopy and quantified using fluorescent spectroscopy. The methods are simple and can provide complementary functional and structural information in studies of the effects of embryotoxicants on yolk sac function.
组织营养性营养是一个过程，通过该过程啮齿动物的内脏卵黄囊（VYS）内化外源性大分子，将它们降解，并将降解产物输送到胚胎用于从头进行大分子生物合成。在功能性胎盘形成之前的妊娠早期，这个过程对胚胎发育很重要。组织营养性营养的定量和可视化可以使用荧光示踪分子来完成，例如异硫氰酸荧光素偶联白蛋白（FITC-白蛋白），它可以通过荧光显微镜进行可视化，并使用荧光光谱进行定量。这些方法很简单，并且在胚胎毒性物质对卵黄囊功能影响的研究中可以提供互补的功能和结构信息。
