Cheng Yuanzhi, Xu Tianjun, Jin Xiaoxiao, Shi Ge, Wang Rixin
Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan 316000, PR China.
Mol Biol (Mosk). 2012 Mar-Apr;46(2):224-33.
The complete mitochondrial genome sequence of the silver croaker, Argyrosomus argentatus, was obtained by using LA-PCR and sequencing. The mitogenome is 16485 bp in length, consists of 13 protein-coding genes, two ribosomal RNAs, 22 transfer RNAs, and a non-coding control region like those found in other vertebrates, with the gene order similar to that of typical teleosts. Most of the genes of A. argentatus were encoded on the H-strand, while the ND6 and eight tRNA (Gln, Ala, Asn, Cys, Tyr, Ser (UCN), Glu and Pro)) genes were encoded on the L-strand. The reading frames of two pairs of genes overlapped: ATPase8 and 6 and ND4L and ND4 by ten and seven nucleotides, respectively. The origin of L-strand replication in A. argentatus was in a cluster of five tRNA genes (WANCY) and was 46 nucleotides in length. The conserved motif (5'-GCGGG-3') was found at the base of the stem within the tRNA(Cys) gene. Within the control region, we identified all of the conserved motifs except for CSB-F.
通过长片段PCR（LA-PCR）和测序获得了银鲈（Argyrosomus argentatus）的完整线粒体基因组序列。该线粒体基因组长度为16485 bp，由13个蛋白质编码基因、两个核糖体RNA、22个转运RNA和一个非编码控制区组成，与其他脊椎动物中的情况类似，其基因排列顺序与典型硬骨鱼类相似。银鲈的大多数基因在重链（H链）上编码，而ND6和8个tRNA（Gln、Ala、Asn、Cys、Tyr、Ser（UCN）、Glu和Pro）基因在轻链（L链）上编码。两对基因的阅读框存在重叠：ATPase8和6以及ND4L和ND4分别重叠10个和7个核苷酸。银鲈L链复制起点位于一组5个tRNA基因（WANCY）中，长度为46个核苷酸。在tRNA（Cys）基因的茎基部发现了保守基序（5'-GCGGG-3'）。在控制区内，除了CSB-F外，我们鉴定出了所有保守基序。
