Department of Periodontology, Faculty of Dental Sciences, Sri Ramachandra University, Chennai, India.
Oral Dis. 2012 Nov;18(8):778-85. doi: 10.1111/j.1601-0825.2012.01945.x. Epub 2012 Jun 4.
The aim of this study was to evaluate the presence and distribution of CD1a and S100 protein markers in states of gingival health and chronic periodontitis in human subjects.
Gingival tissue samples were derived from 10 healthy and 10 chronic periodontitis-affected human subjects. The presence and distribution of CD1a and S100 protein was assessed using immunohistochemistry, and the cell types involved in their expression was determined.
The presence and distribution of CD1a was confined only to the gingival epithelium, whereas S100 was seen in the epithelium and connective tissue. However, increased expression of both CD1a and S100 protein was seen in periodontitis-affected gingival tissues compared with healthy gingiva. Immunohistochemistry demonstrated that CD1a- and S100-positive cells in the epithelium are Langerhans cells (LCs) and S100 positive cells in the connective tissue are dendritic cells (DCs).
Our findings suggest the transition of CD1a-positive LCs to S100-positive DCs from epithelium to connective tissue in response to an antigenic challenge. Demonstration of increased number of S100-positive DCs in the gingival connective tissue in chronic periodontitis possibly suggests their involvement in bone resorption in addition to their antigen presentation property.
本研究旨在评估人类牙龈健康和慢性牙周炎状态下 CD1a 和 S100 蛋白标志物的存在和分布。
从 10 名健康和 10 名慢性牙周炎患者中获取牙龈组织样本。使用免疫组织化学评估 CD1a 和 S100 蛋白的存在和分布，并确定其表达涉及的细胞类型。
CD1a 的存在和分布仅局限于牙龈上皮，而 S100 则见于上皮和结缔组织。然而，与健康牙龈相比，牙周炎受累的牙龈组织中 CD1a 和 S100 蛋白的表达增加。免疫组织化学显示，上皮中 CD1a 和 S100 阳性细胞为朗格汉斯细胞（LCs），结缔组织中 S100 阳性细胞为树突状细胞（DCs）。
我们的发现表明，在抗原刺激下，上皮中 CD1a 阳性 LCs 向结缔组织中 S100 阳性 DC 的转化。在慢性牙周炎中，牙龈结缔组织中 S100 阳性 DC 的数量增加，这可能表明它们除了具有抗原呈递特性外，还参与了骨吸收。
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