Crawford J M, Krisko J M, Morris G A, Chambers D A
Center for Research in Periodontal Diseases and Oral Molecular Biology, University of Illinois, Chicago 60612.
Reg Immunol. 1989 Mar-Apr;2(2):91-7.
The distribution of CD1a antigen in gingival epithelium of clinically healthy gingiva was examined and compared with the distribution in gingival epithelium of adult periodontitis lesions. Cryostat sections were examined with monoclonal antibodies to CD1a antigen using the ABC immunoperoxidase technique. In healthy gingiva, CD1a was limited to Langerhans cells (LC) which were observed throughout the length of the external epithelium and orosulcular epithelium. The numbers of LC expressed either per unit length of orosulcular epithelium or per mm2 were similar to the numbers in external gingiva. Junctional epithelium contained few if any dendritic LC. The numbers of LC in pocket epithelium of adult periodontitis lesions were significantly lower compared with orosulcular epithelium of healthy tissue and compared with external gingiva of diseased tissue (p less than 0.005). In many sections, no LC were identified in pocket epithelium. In 5 of 8 adult periodontitis sites, CD1a was also observed in association with the membranes of suprabasal keratinocytes in external and pocket epithelium in areas where no LC were identified. These findings provide further evidence that changes in gingival epithelial cells occur in periodontal disease which are analogous to those documented in dermatological diseases and suggest that epithelium may play a role in gingival homeostasis and in the pathogenesis of periodontal diseases.
检测了临床健康牙龈的牙龈上皮中CD1a抗原的分布，并与成人牙周炎病变的牙龈上皮中的分布进行了比较。使用ABC免疫过氧化物酶技术，用抗CD1a抗原的单克隆抗体检查低温恒温器切片。在健康牙龈中，CD1a仅限于朗格汉斯细胞（LC），这些细胞在外上皮和龈沟上皮的全长中均有观察到。龈沟上皮每单位长度或每平方毫米表达的LC数量与外牙龈中的数量相似。结合上皮中几乎没有树突状LC。与健康组织的龈沟上皮相比，以及与患病组织的外牙龈相比，成人牙周炎病变的袋上皮中的LC数量显著降低（p小于0.005）。在许多切片中，袋上皮中未发现LC。在8个成人牙周炎部位中的5个部位，在未发现LC的区域，外上皮和袋上皮中基底上角质形成细胞的膜也观察到CD1a。这些发现进一步证明，牙周疾病中牙龈上皮细胞会发生变化，这与皮肤病中记录的变化类似，并表明上皮可能在牙龈稳态和牙周疾病的发病机制中起作用。
