Medical Scientist Training Program, Baylor College of Medicine, Houston, Texas 77030, USA.
Microcirculation. 2012 Oct;19(7):652-63. doi: 10.1111/j.1549-8719.2012.00200.x.
One important contributor to tissue graft viability is angiogenic maturation of the graft tissue bed. This study uses scale-invariant microvascular morphological quantification to track vessel maturation and remodeling in a split-thickness skin-grafting model over 21 days, comparing the results to classical techniques.
Images from a previous study of split-thickness skin grafting in rats were analyzed. Microvascular morphology (fractal and multifractal dimensions, lacunarity, and vessel density) within fibrin interfaces of samples over time was quantified using classical semi-automated methods and automated multifractal and lacunarity analyses.
Microvessel morphology increased in density and complexity, from three to seven days after engraftment and then regressed by 21 days. Vessel density increased from 0.07 on day 3 to 0.20 on day 7 and then decreased to 0.06 on day 21. A similar trend was seen for the fractal dimension that increased from 1.56 at three days to 1.77 at seven days then decreased to 1.57 by 21 days. Vessel diameters did not change whereas complexity and density did, signaling remodeling.
This new automated analysis identified design parameters for tissue engraftment and could be used in other models of graft vessel biology to track proliferation and pruning of complex vessel beds.
组织移植物活力的一个重要贡献因素是移植物组织床的血管生成成熟。本研究使用标度不变的微血管形态定量方法，在 21 天的时间内跟踪全厚皮片移植模型中的血管成熟和重塑，将结果与经典技术进行比较。
对大鼠全厚皮片移植的先前研究中的图像进行了分析。使用经典的半自动方法和自动多重分形和空隙度分析，对样本中纤维蛋白界面内的微血管形态（分形和多重分形维数、空隙度和血管密度）随时间的变化进行了定量。
微血管形态的密度和复杂性增加，在移植后 3 至 7 天增加，然后在 21 天减少。血管密度从第 3 天的 0.07 增加到第 7 天的 0.20，然后减少到第 21 天的 0.06。分形维数也呈现出相似的趋势，从第 3 天的 1.56 增加到第 7 天的 1.77，然后减少到第 21 天的 1.57。血管直径没有变化，而复杂性和密度发生了变化，表明发生了重塑。
这种新的自动分析方法确定了组织移植的设计参数，可用于其他移植物血管生物学模型中，以跟踪复杂血管床的增殖和修剪。
