Watanabe Maiko, Goto Keiichi, Sugita-Konishi Yoshiko, Kamata Yoichi, Hara-Kudo Yukiko
Division of Microbiology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, Japan. :
J Vet Med Sci. 2012 Oct;74(10):1333-6. doi: 10.1292/jvms.12-0034. Epub 2012 May 25.
We developed a new system for detection of whole-genome differentiation using DNA-DNA hybridization, and tested its sensitivity with three closely-related Fusarium species. We compared DNA-DNA relatedness to nucleotide sequence homologies of five genetic regions between each of five strains of three Fusarium species. DNA-DNA relatedness by our system was 16.2-86.6%. Sequence homologies of 18S rDNA, rDNA cluster region from ITS1 to 28S rDNA, β-tub, EF-1α and lys2 were 100.0, 99.0-100.0, 96.7-100.0, 95.1-99.4, and 94.7-100.0%, respectively. Our system could clearly detect differentiation between closely-related fungal species which have very similar morphological-characteristics, and exhibit little diagnoses in nucleotide sequences. Our results suggest that this system is a good tool for identification and phylogenetic analysis of closely-related fungal species.
我们开发了一种利用DNA-DNA杂交检测全基因组差异的新系统，并使用三种密切相关的镰刀菌属物种测试了其灵敏度。我们比较了三种镰刀菌属物种的五个菌株中每两个菌株之间五个遗传区域的DNA-DNA相关性与核苷酸序列同源性。我们系统检测的DNA-DNA相关性为16.2-86.6%。18S rDNA、从ITS1到28S rDNA的rDNA簇区域、β-微管蛋白、延伸因子1α和lys2的序列同源性分别为100.0%、99.0-100.0%、96.7-100.0%、95.1-99.4%和94.7-100.0%。我们的系统能够清晰地检测出形态特征非常相似且核苷酸序列诊断差异很小的密切相关真菌物种之间的差异。我们的结果表明，该系统是密切相关真菌物种鉴定和系统发育分析的良好工具。
