Matsumura Shigeru, Toyoshima Fumiko
Department of Cell Biology, Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan.
Cell Struct Funct. 2012;37(2):81-7. doi: 10.1247/csf.12011. Epub 2012 May 19.
Directing the axis of cell division toward extrinsic and intrinsic cues plays a fundamental role in morphogenesis, asymmetric cell division, and stem cell self-renewal. Recent studies highlight the misorientation of the cell division axis as a cause of mammalian diseases, including polycystic kidney disease. Although the core regulators for oriented cell division have been identified in invertebrate model systems, we still have an imprecise picture of the relevant signaling networks in the mammalian system. The reasons for this include the lack of established approaches in mammalian cells to survey the molecules required for the spindle orientation. Here we summarize our recent study on a genome-scale RNA-mediated interference screen of human kinases to identify a new player for the oriented cell division in both culture cells and developing mammalian tissues.
将细胞分裂轴导向外在和内在信号在形态发生、不对称细胞分裂和干细胞自我更新中起着基础性作用。最近的研究强调细胞分裂轴方向错误是包括多囊肾病在内的哺乳动物疾病的一个病因。尽管在无脊椎动物模型系统中已确定了定向细胞分裂的核心调节因子，但我们对哺乳动物系统中相关信号网络的了解仍不精确。其原因包括在哺乳动物细胞中缺乏用于检测纺锤体定向所需分子的既定方法。在此，我们总结了我们最近关于对人类激酶进行全基因组规模RNA介导的干扰筛选的研究，以确定培养细胞和发育中的哺乳动物组织中定向细胞分裂的一个新参与者。
